| RRC ID |
147
|
| Author |
Sato Y, Ajiki T, Inoue S, Fujishiro J, Yoshino H, Igarashi Y, Hakamata Y, Kaneko T, Murakami T, Kobayashi E.
|
| Title |
Gene silencing in rat-liver and limb grafts by rapid injection of small interference RNA.
|
| Journal |
Transplantation
|
| Abstract |
Genetic modification is a promising therapeutic strategy for organ transplantation. In previous studies, we introduced a nonviral method of gene transfer to donor grafts using an organ-selective injection technique to up-regulate gene expression. Based on the attractive methodology of RNA interference for silencing a particular gene expression, we applied our catheter-based injection method to transfer small interference RNA (siRNA)-green fluorescence protein (GFP) into liver and limb grafts. We first quantified the interfering activity after the systemic delivery of siRNA in the liver of Alb-DsRed2 transgenic (Tg) rats using in vivo bioimaging system. Then, using GFP Tg Lewis rats as donors, transient down-regulation of the GFP expression was achieved both in liver- and limb-transplantation models by the preoperative rapid injection of siRNA. Genetic modification by siRNA may provide new therapeutic options for down-regulation of endogenous antigenicity.
|
| Volume |
79(2)
|
| Pages |
240-3
|
| Published |
2005-1-27
|
| DOI |
10.1097/01.tp.0000147786.52502.2f
|
| PII |
00007890-200501270-00017
|
| PMID |
15665774
|
| MeSH |
Animals
Animals, Genetically Modified
Gene Silencing*
Genes, Reporter
Green Fluorescent Proteins / genetics
Injections
Liver Transplantation / physiology*
RNA, Small Interfering / administration & dosage
RNA, Small Interfering / genetics*
Rats
|
| IF |
4.546
|
| Times Cited |
25
|
|
WOS Category
|
SURGERY
TRANSPLANTATION
IMMUNOLOGY
|
| Resource |
| Rats |
|
