RRC ID |
147
|
著者 |
Sato Y, Ajiki T, Inoue S, Fujishiro J, Yoshino H, Igarashi Y, Hakamata Y, Kaneko T, Murakami T, Kobayashi E.
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タイトル |
Gene silencing in rat-liver and limb grafts by rapid injection of small interference RNA.
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ジャーナル |
Transplantation
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Abstract |
Genetic modification is a promising therapeutic strategy for organ transplantation. In previous studies, we introduced a nonviral method of gene transfer to donor grafts using an organ-selective injection technique to up-regulate gene expression. Based on the attractive methodology of RNA interference for silencing a particular gene expression, we applied our catheter-based injection method to transfer small interference RNA (siRNA)-green fluorescence protein (GFP) into liver and limb grafts. We first quantified the interfering activity after the systemic delivery of siRNA in the liver of Alb-DsRed2 transgenic (Tg) rats using in vivo bioimaging system. Then, using GFP Tg Lewis rats as donors, transient down-regulation of the GFP expression was achieved both in liver- and limb-transplantation models by the preoperative rapid injection of siRNA. Genetic modification by siRNA may provide new therapeutic options for down-regulation of endogenous antigenicity.
|
巻・号 |
79(2)
|
ページ |
240-3
|
公開日 |
2005-1-27
|
DOI |
10.1097/01.tp.0000147786.52502.2f
|
PII |
00007890-200501270-00017
|
PMID |
15665774
|
MeSH |
Animals
Animals, Genetically Modified
Gene Silencing*
Genes, Reporter
Green Fluorescent Proteins / genetics
Injections
Liver Transplantation / physiology*
RNA, Small Interfering / administration & dosage
RNA, Small Interfering / genetics*
Rats
|
IF |
4.546
|
引用数 |
25
|
WOS 分野
|
SURGERY
TRANSPLANTATION
IMMUNOLOGY
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リソース情報 |
ラット |
|