Reference - Detail
| RRC ID | 148 |
|---|---|
| Author | Satoh M, Haruta-Satoh E, Omori A, Oh-Ishi M, Kodera Y, Furudate S, Maeda T. |
| Title | Effect of thyroxine on abnormal pancreatic proteomes of the hypothyroid rdw rat. |
| Journal | Proteomics |
| Abstract |
A mutation in the thyroglobulin (Tg) gene is the primary cause of hereditary dwarfism and hypothyroidism in the rdw rat. Despite the Tg mutation that causes a Tg shortage, rdw rats survive. The present study examines the influences of this condition on the pancreatic proteome. Normal control (group 1; n = 19) and rdw rats that did not receive L-thyroxine (T4) (group 2; n = 27) were sacrificed from 4 to 56 weeks after birth. The rdw rats were supplemented either with daily intraperitoneal injections of T4 from 3 to 28 days after birth (group 3; n = 4) or with normal thyroid tissues grafted at 4 weeks of age (group 4; n = 3). Groups 3 and 4 were sacrificed 12 weeks after birth. Pancreatic proteomes analyzed by two-dimensional gel electrophoresis showed that levels of at least four pancreatic proteins were higher in group 2 than in group 1, and that those of four were lower. Cluster decomposition and principal component analysis of the eight protein contents showed that groups 1 and 2 were separated into two clusters and that pancreatic proteomes of group 4 were better normalized than those of group 3. Injecting T4 into group 3 was temporarily effective, whereas the thyroid graft to group 4 provided a continuous positive effect, which concurred with the increased body weight of the other two groups of rdw rats that received grafts of normal thyroids. |
| Volume | 5(4) |
| Pages | 1113-24 |
| Published | 2005-3-1 |
| DOI | 10.1002/pmic.200401117 |
| PMID | 15712237 |
| MeSH | Animals Body Weight Cluster Analysis Disease Models, Animal Electrophoresis, Gel, Two-Dimensional Female Genetic Markers Growth Hormone / metabolism Heterozygote Hypothyroidism / metabolism* Image Processing, Computer-Assisted Male Mass Spectrometry Multivariate Analysis Mutation Pancreas / drug effects* Pancreas / metabolism* Proteomics / methods* Rats Rats, Mutant Strains Receptors, Thyroid Hormone / metabolism Sequence Analysis, Protein Thyroid Gland / metabolism Thyroxine / pharmacology* Time Factors |
| IF | 3.254 |
| Times Cited | 18 |
| WOS Category | BIOCHEMICAL RESEARCH METHODS BIOCHEMISTRY & MOLECULAR BIOLOGY |
| Altmetric score |
オルトメトリクス指標項目
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| Total number of mentions | 0 |
| Resource | |
| Rats | WIC-Tgrdw/Kts(strainID=193) |