論文 - 詳細
| RRC ID | 1498 |
|---|---|
| 著者 | Kawai S, Kato T, Inaba H, Okahashi N, Amano A. |
| タイトル | Odd-skipped related 2 splicing variants show opposite transcriptional activity. |
| ジャーナル | Biochem Biophys Res Commun |
| Abstract |
Odd-skipped related 2 (Osr2) is expressed in the domain of epithelial-mesenchymal interactions during tooth and kidney development. In this study, we report that alternative splicing of exon 4 resulted in two transcripts, Osr2A and Osr2B. These variants utilized a different splice acceptor, and Osr2B exhibited a frameshift followed by an early stop codon. Osr2A mRNA produced a protein that was 36 amino acids longer than Osr2B at the C-terminus and had five zinc-finger domains, whereas Osr2B had three zinc-finger motives. The 2 Osr2 variants were found in kidney, skeletal muscle, and testis, and Osr2B was predominantly transcribed in each. Flag-tagged variants showed the same localization in cell nuclei, however, Gal4 fusion proteins showed opposite transcriptional activities. Further, Flag-tagged variants also showed opposite transcriptional activities, though they were in contrast to the findings for the Gal4-fused variants. Our results indicate that Osr2 bifurcates its function by alternative splicing. |
| 巻・号 | 328(1) |
| ページ | 306-11 |
| 公開日 | 2005-3-4 |
| DOI | 10.1016/j.bbrc.2004.12.173 |
| PII | S0006-291X(04)02976-6 |
| PMID | 15670784 |
| MeSH | Animals COS Cells Chlorocebus aethiops Gene Expression Regulation / genetics* Genetic Variation / genetics Mice Protein Splicing / genetics* Subcellular Fractions / metabolism Transcription Factors / genetics* Transcription Factors / metabolism* Transcriptional Activation / genetics* |
| IF | 2.985 |
| 引用数 | 19 |
| WOS 分野 | BIOPHYSICS BIOCHEMISTRY & MOLECULAR BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | Wikipedia |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | 10T1/2(RCB0247) |