| RRC ID |
1505
|
| 著者 |
Awata T, Kurihara S, Takata N, Neda T, Iizuka H, Ohkubo T, Osaki M, Watanabe M, Nakashima Y, Inukai K, Inoue I, Kawasaki I, Mori K, Yoneya S, Katayama S.
|
| タイトル |
Functional VEGF C-634G polymorphism is associated with development of diabetic macular edema and correlated with macular retinal thickness in type 2 diabetes.
|
| ジャーナル |
Biochem Biophys Res Commun
|
| Abstract |
Since vascular endothelial growth factor (VEGF) has a strong effect on induction of vascular permeability, VEGF is an attractive candidate gene for development of diabetic macular edema (ME). Among the 378 patients with type 2 diabetes studied, 203 patients had no retinopathy, 93 had non-proliferative diabetic retinopathy (NPDR), and 82 had proliferative diabetic retinopathy (PDR). ME was present in 16 patients with NPDR and 47 patients with PDR. We genotyped three VEGF polymorphisms: C-2,578A, G-1,154A, and C-634G. Genotype and allele distribution of C-634G, but not C-2,578A or G-1,154A, were significantly different between patients with and without diabetic retinopathy. Logistic regression analysis revealed that the C-634G genotype was a risk factor for DR (p = 0.002), and furthermore for ME (p = 0.047), independently from severity of DR, with the -634C allele increasing the risk. Macular thickness measured by optical coherence tomography was correlated with the C-634G genotype, with the trend increasing with the presence of more -634C alleles (p = 0.006). Stepwise regression analysis showed that duration of diabetes and presence of the C-634G genotype were independent predictors of macular thickness. In addition, basic transcriptional activity levels associated with the -634C allele were greater compared to those seen with the -634G allele in human glioma and lymphoblastic T-lymphocyte cells. These results demonstrate that the VEGF C-634G polymorphism is a genetic risk factor for ME as well as DR.
|
| 巻・号 |
333(3)
|
| ページ |
679-85
|
| 公開日 |
2005-8-5
|
| DOI |
10.1016/j.bbrc.2005.05.167
|
| PII |
S0006-291X(05)01168-X
|
| PMID |
15963467
|
| MeSH |
Adult
Aged
Base Sequence
DNA Primers
Diabetes Mellitus, Type 2 / genetics
Diabetes Mellitus, Type 2 / pathology*
Diabetic Retinopathy / genetics
Female
Genetic Predisposition to Disease
Humans
Macular Edema / genetics*
Male
Middle Aged
Polymorphism, Genetic*
Vascular Endothelial Growth Factor A / genetics*
|
| IF |
2.985
|
| 引用数 |
99
|
|
WOS 分野
|
BIOPHYSICS
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| リソース情報 |
| ヒト・動物細胞 |
GI-1(RCB0763)
Jurkat(RCB0806) |
