論文 - 詳細
| RRC ID | 1514 |
|---|---|
| 著者 | Nagaoka Y, Otsu K, Okada F, Sato K, Ohba Y, Kotani N, Fujii J. |
| タイトル | Specific inactivation of cysteine protease-type cathepsin by singlet oxygen generated from naphthalene endoperoxides. |
| ジャーナル | Biochem Biophys Res Commun |
| Abstract |
Singlet oxygen is a causal factor in light-induced skin photoaging and the cytotoxic process of tumor cells in photodynamic chemotherapy. To develop a better understanding of the functional consequences of protein modification by singlet oxygen, the effects of naphthalene endoperoxide on lysosomal protease, cathepsin, were examined. When the soluble fraction of normal human fetal skin fibroblast cells was treated with the endoperoxide, the activities of cysteine proteases, cathepsins B and L/S, were inhibited, but that of aspartate protease, cathepsin D/E, was not. The reduction of the endoperoxide-treated soluble fractions by treatment with dithiothreitol barely recovered the activities. Cathepsin B, purified from normal human liver, exhibited similar profiles to that in cytosol. These data suggest that singlet oxygen oxidatively modifies an amino acid residue essential for catalysis and consequently results in the irreversible inactivation of cysteine protease-type cathepsin. |
| 巻・号 | 331(1) |
| ページ | 215-23 |
| 公開日 | 2005-5-27 |
| DOI | 10.1016/j.bbrc.2005.03.146 |
| PII | S0006-291X(05)00649-2 |
| PMID | 15845381 |
| MeSH | Cathepsin B / metabolism Cathepsin L Cathepsins / metabolism* Cell Line Cysteine Endopeptidases / metabolism* Humans Lipid Peroxidation / drug effects Lipid Peroxides / chemistry Lipid Peroxides / pharmacology Naphthalenes / chemistry Naphthalenes / pharmacology* Peroxides / chemistry Peroxides / pharmacology* Propionates / chemistry Propionates / pharmacology Reactive Nitrogen Species / metabolism Reactive Oxygen Species / metabolism Singlet Oxygen / metabolism* |
| IF | 2.985 |
| 引用数 | 38 |
| WOS 分野 | BIOPHYSICS BIOCHEMISTRY & MOLECULAR BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 2 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | HFSKF-II(RCB0698) |