論文 - 詳細
| RRC ID | 1515 |
|---|---|
| 著者 | Tsuji AB, Sudo H, Sugyo A, Otsuki M, Miyagishi M, Taira K, Imai T, Harada YN. |
| タイトル | A fast, simple method for screening radiation susceptibility genes by RNA interference. |
| ジャーナル | Biochem Biophys Res Commun |
| Abstract |
Radiotherapy can cause unacceptable levels of damage to normal tissues in some cancer patients. To understand the molecular mechanisms underlying radiation-induced physiological responses, and to be able to predict the radiation susceptibility of normal tissues in individual patients, it is important to identify a comprehensive set of genes responsible for radiation susceptibility. We have developed a simple and rapid 96-well screening protocol using cell proliferation assays and RNA interference to identify genes associated with radiation susceptibility. We evaluated the performance of alamarBlue-, BrdU-, and sulforhodamine B-based cell proliferation assays using the 96-well format. Each proliferation assay detected the known radiation susceptibility gene, PRKDC. In a trial screen using 28 shRNA vectors, another known gene, CDKN1A, and one new radiation susceptibility gene, ATP5G3, were identified. Our results indicate that this method may be useful for large-scale screens designed to identify novel radiation susceptibility genes. |
| 巻・号 | 333(4) |
| ページ | 1370-7 |
| 公開日 | 2005-8-12 |
| DOI | 10.1016/j.bbrc.2005.06.047 |
| PII | S0006-291X(05)01265-9 |
| PMID | 15979584 |
| MeSH | Cell Count / methods* Cell Survival / radiation effects* Disease Susceptibility / diagnosis Dose-Response Relationship, Drug Gene Expression Profiling / methods* Gene Silencing* HeLa Cells Humans RNA Interference* Radiation Dosage Radiation Injuries / diagnosis Radiation Injuries / etiology Radiation Injuries / genetics* Radiation Tolerance / genetics* Radiation, Ionizing Risk Assessment / methods* Risk Factors |
| IF | 2.985 |
| 引用数 | 11 |
| WOS 分野 | BIOPHYSICS BIOCHEMISTRY & MOLECULAR BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | HeLa(RCB0007) |