論文 - 詳細
| RRC ID | 1518 |
|---|---|
| 著者 | Chinen A, Matsumoto Y, Kawamura S. |
| タイトル | Reconstitution of ancestral green visual pigments of zebrafish and molecular mechanism of their spectral differentiation. |
| ジャーナル | Mol Biol Evol |
| Abstract |
We previously reported that zebrafish have four tandemly duplicated green (RH2) opsin genes (RH2-1, RH2-2, RH2-3, and RH2-4). Absorption spectra vary widely among the four photopigments reconstituted with 11-cis retinal, with their peak absorption spectra (lambda(max)) being 467, 476, 488, and 505 nm, respectively. In this study, we inferred the ancestral amino acid (aa) sequences of the zebrafish RH2 opsins by likelihood-based Bayesian statistics and reconstituted the ancestral opsins by site-directed mutagenesis. The ancestral pigment (A1) to the four zebrafish RH2 pigments and that (A3) to RH2-3 and RH2-4 showed lambda(max) at 506 nm, while that (A2) to RH2-1 and RH2-2 showed a lambda(max) at 474 nm, indicating that a spectral shift had occurred toward the shorter wavelength on the evolutionary lineages A1 to A2 by 32 nm, A2 to RH2-1 by 7 nm, and A3 to RH2-3 by 18 nm. Pigment chimeras and site-directed mutagenesis revealed a large contribution (approximately 15 nm) of glutamic acid to glutamine substitution at residue 122 (E122Q) to the A1 to A2 and A3 to RH2-3 spectral shifts. However, the remaining spectral differences appeared to result from complex interactive effects of a number of aa replacements, each of which has only a minor spectral contribution (1-3 nm). The four zebrafish RH2 pigments cover nearly an entire range of lambda(max) distribution among vertebrate RH2 pigments and provide an excellent model to study spectral tuning mechanisms of RH2 in vertebrates. |
| 巻・号 | 22(4) |
| ページ | 1001-10 |
| 公開日 | 2005-4-1 |
| DOI | 10.1093/molbev/msi086 |
| PII | msi086 |
| PMID | 15647516 |
| MeSH | Amino Acid Sequence Animals Bayes Theorem Molecular Sequence Data Mutation Retinal Pigments / chemistry* Retinal Pigments / genetics Sequence Homology, Amino Acid Spectrum Analysis Zebrafish / genetics* |
| IF | 11.062 |
| 引用数 | 40 |
| WOS 分野 | GENETICS & HEREDITY BIOCHEMISTRY & MOLECULAR BIOLOGY EVOLUTIONARY BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 2 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | COS-1(RCB0143) |