| RRC ID |
1519
|
| Author |
Akimoto T, Ushida T, Miyaki S, Akaogi H, Tsuchiya K, Yan Z, Williams RS, Tateishi T.
|
| Title |
Mechanical stretch inhibits myoblast-to-adipocyte differentiation through Wnt signaling.
|
| Journal |
Biochem Biophys Res Commun
|
| Abstract |
Myoblasts are able to differentiate into other mesenchymal lineages including adipocytes and osteoblasts. However, it is not known how this differentiation is normally regulated in intact animals and humans. Here, we subjected cultured C2C12 myoblasts to cyclic mechanical stretch (20% elongation) during differentiation into adipocytes. Mechanical stretch inhibited the myoblast-to-adipocyte differentiation significantly, concurrent with an enhanced expression of Wnt10b mRNA. Inhibition of the Wnt signaling by incubation of the myoblasts with a soluble Wnt ligand, sFRP-2, abolished the inhibitory function of mechanical stretch on adipogenesis. These findings provide evidence that mechanical stretch inhibits myoblast-to-adipocyte differentiation possibly through Wnt signaling.
|
| Volume |
329(1)
|
| Pages |
381-5
|
| Published |
2005-4-1
|
| DOI |
10.1016/j.bbrc.2005.01.136
|
| PII |
S0006-291X(05)00122-1
|
| PMID |
15721317
|
| MeSH |
Adipocytes / cytology*
Adipocytes / metabolism
Animals
Azo Compounds / pharmacology
Cell Differentiation
Cell Line
Cell Lineage
Cell Membrane / metabolism
Intercellular Signaling Peptides and Proteins / metabolism*
Ligands
Mice
Myoblasts / cytology*
RNA / metabolism
RNA, Messenger / metabolism
Reverse Transcriptase Polymerase Chain Reaction
Signal Transduction
Stress, Mechanical
Wnt Proteins
|
| IF |
2.985
|
| Times Cited |
47
|
|
WOS Category
|
BIOPHYSICS
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| Resource |
| Human and Animal Cells |
C2C12(RCB0987) |
