| RRC ID |
15297
|
| 著者 |
Kiuchi T, Banno Y, Katsuma S, Shimada T.
|
| タイトル |
Mutations in an amino acid transporter gene are responsible for sex-linked translucent larval skin of the silkworm, Bombyx mori.
|
| ジャーナル |
Insect Biochem Mol Biol
|
| Abstract |
The sex-linked translucent (os) mutation in the silkworm, Bombyx mori, confers slightly translucent larval skin resulting from a decrease in the incorporation of uric acid into epidermal cells. By positional cloning, we narrowed a region linked to the os phenotype to approximately 157 kb located on scaffold Bm_scaf72 on the Z chromosome (chromosome 1). The region contained four gene models. Sequencing analysis revealed that one of the candidate genes had a 7-bp deletion in the coding region. We also found a 111-bp deletion or single-nucleotide substitution in the same gene using independent os mutant strains. Because all the mutations caused the generation of abnormal transcripts followed by translation of a truncated protein, we conclude that the mutation of this candidate gene is responsible for the translucent larval skin of the os mutant. Sequence analysis indicated that the gene responsible for the os mutation had homology to amino acid transporters of the solute carrier family of proteins. Our results suggest that solute carrier proteins are involved in uric acid transport in insects and other invertebrates.
|
| 巻・号 |
41(9)
|
| ページ |
680-7
|
| 公開日 |
2011-9-1
|
| DOI |
10.1016/j.ibmb.2011.04.011
|
| PII |
S0965-1748(11)00100-7
|
| 解説 |
os(伴性油)の原因遺伝子はSolute Carrier Familyに属するアミノ酸トランスポーターに相同性を示すタンパク質をコードしている。K151系統とo03系統のos個体では、os遺伝子のエクソン５の内部に7bpの欠失が存在する。o01系統のos個体では、os遺伝子のエクソン３の３'側に欠失がある。
|
| PMID |
21619931
|
| MeSH |
Amino Acid Transport Systems / genetics*
Amino Acid Transport Systems / metabolism
Animals
Base Sequence
Bombyx / chemistry*
Bombyx / classification
Bombyx / genetics*
Bombyx / metabolism
Cloning, Molecular
Female
Gene Expression
Insect Proteins / genetics*
Insect Proteins / metabolism
Male
Molecular Sequence Data
Mutagenesis
Phylogeny
Sequence Analysis, DNA
Skin / chemistry
Skin / metabolism
Uric Acid / metabolism
|
| IF |
3.827
|
| 引用数 |
16
|
|
WOS 分野
|
ENTOMOLOGY
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| リソース情報 |
| カイコ |
o01
o03
k151 |
