| RRC ID |
15316
|
| Author |
Shahriari M, Richter K, Keshavaiah C, Sabovljevic A, Huelskamp M, Schellmann S.
|
| Title |
The Arabidopsis ESCRT protein-protein interaction network.
|
| Journal |
Plant Mol Biol
|
| Abstract |
In yeast, endosomal sorting of monoubiquitylated transmembrane proteins is performed by a subset of the 19 "class E vacuolar protein sorting" proteins. The core machinery consists of 11 proteins that are organised in three complexes termed ESCRT I-III (endosomal sorting complex required for transport I-III) and is conserved in eukaryotic cells. While the pathway is well understood in yeast and animals, the plant ESCRT system is largely unexplored. At least one sequence homolog for each ESCRT component can be found in the Arabidopsis genome. Generally, sequence conservation between yeast/animals and the Arabidopsis proteins is low. To understand details about participating proteins and complex organization we have performed a systematic pairwise yeast two hybrid analysis of all Arabidopsis proteins showing homology to the ESCRT core machinery. Positive interactions were validated using bimolecular fluorescence complementation. In our experiments, most putative ESCRT components exhibited interactions with other ESCRT components that could be shown to occur on endosomes suggesting that despite their low homology to their yeast and animal counterparts they represent functional components of the plant ESCRT pathway.
|
| Volume |
76(1-2)
|
| Pages |
85-96
|
| Published |
2011-5-1
|
| DOI |
10.1007/s11103-011-9770-4
|
| PMID |
21442383
|
| MeSH |
Animals
Arabidopsis / cytology
Arabidopsis / genetics
Arabidopsis / metabolism*
Arabidopsis Proteins / genetics
Arabidopsis Proteins / metabolism*
Computer Simulation
Endosomal Sorting Complexes Required for Transport / metabolism*
Luminescent Proteins / genetics
Luminescent Proteins / metabolism
Microscopy, Confocal
Models, Biological
Protein Binding
Protein Interaction Mapping / methods*
Protoplasts / metabolism
Recombinant Fusion Proteins / genetics
Recombinant Fusion Proteins / metabolism
Saccharomyces cerevisiae / genetics
Signal Transduction
Two-Hybrid System Techniques
|
| IF |
3.302
|
| Times Cited |
31
|
|
WOS Category
|
PLANT SCIENCES
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| Resource |
| Arabidopsis / Cultured plant cells, genes |
pda04818
pda09962
pda06592
pda08063
pda03672
pda11165
pda02568
pda02482
pda01247
pda02381
pdx96416
pdx96789
pdy35572
pda02252
pdx36377
pdx95869
pdx99743 |
