| RRC ID |
1531
|
| 著者 |
Katayama H, Yokota H, Akao T, Nakamura O, Ohba M, Mekada E, Mizuki E.
|
| タイトル |
Parasporin-1, a novel cytotoxic protein to human cells from non-insecticidal parasporal inclusions of Bacillus thuringiensis.
|
| ジャーナル |
J Biochem
|
| Abstract |
Pro-parasporin-1 is a parasporal inclusion protein of the non-insecticidal Bacillus thuringiensis strain A1190. Cytotoxic fragments, named parasporin-1, were generated from pro-parasporin-1 by trypsin digestion. Parasporin-1 was purified by a combination of chromatography procedures based on the cytotoxic activity to HeLa cells. Two different fragments of 15-kDa and 56-kDa were detected in the purified parasporin-1 fraction. These fragments were tightly associated with each other and could not be separated by chromatography under conditions that preserve cytotoxic activity, indicating that the active form of parasporin-1 is a heterodimer of the 15- and 56-kDa fragments. Amino acid sequencing and MALDI-TOF mass spectrometric analysis revealed that parasporin-1 is generated from pro-parasporin-1 by trypsin digestion at Arg 93 and Arg 231. Of 12 human cell lines tested, parasporin-1 showed strong cytotoxicity to four cell lines derived from cancer tissues, but low to no cytotoxicity to the other cell lines. The time-courses of cytotoxicity indicated that the mode of action of parasporin-1 to sensitive cells differs from that shown for previously isolated cytotoxic proteins from Bacillus thuringiensis, Cyt proteins, and other bacterial pore-forming toxins. Thus, parasporin-1 is a novel cytotoxic protein to human cancer cells produced by B. thuringiensis, and may be useful as a tool to recognize and destroy specific cancer cells.
|
| 巻・号 |
137(1)
|
| ページ |
17-25
|
| 公開日 |
2005-1-1
|
| DOI |
10.1093/jb/mvi003
|
| PII |
137/1/17
|
| PMID |
15713879
|
| MeSH |
Animals
Bacillus thuringiensis / chemistry*
Bacterial Proteins / chemistry
Bacterial Proteins / isolation & purification
Bacterial Proteins / toxicity*
Bacterial Toxins / chemistry
Bacterial Toxins / isolation & purification
Bacterial Toxins / toxicity*
Cell Line
Chlorocebus aethiops
Endotoxins / chemistry
Endotoxins / isolation & purification
Endotoxins / toxicity*
Humans
Mice
|
| IF |
2.476
|
| 引用数 |
48
|
|
WOS 分野
|
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| リソース情報 |
| ヒト・動物細胞 |
HeLa(RCB0007)
Hep G2(RCB1648)
Jurkat(RCB0806)
Sawano(RCB1152)
HL60(RCB0041)
NIH3T3-3
MOLT-4(RCB0206)
A549(RCB0098)
CACO-2(RCB0988)
MRC-5(RCB0218)
COS-7(RCB0539)
Vero(RCB0001) |
