RRC ID 15340
Author Kuromori T, Sugimoto E, Shinozaki K.
Title Arabidopsis mutants of AtABCG22, an ABC transporter gene, increase water transpiration and drought susceptibility.
Journal Plant J.
Abstract In plants, water vapour is released into the atmosphere through stomata in a process called transpiration. Abscisic acid (ABA) is a key phytohormone that facilitates stomatal closure through its action on guard cells. Recently, ATP-binding cassette (ABC) transporter genes, AtABCG25 and AtABCG40, were shown to be involved in ABA transport and responses. However, the functions of many other AtABCG family genes are still unknown. Here, we identified another ABCG gene (AtABCG22) that is required for stomatal regulation in Arabidopsis. The atabcg22 mutant plants had lower leaf temperatures and increased water loss, implying elevated transpiration through an influence on stomatal regulation. We also found that atabcg22 plants were more suspectible to drought stress than wild-type plants. AtABCG22 was expressed in aerial organs, mainly guard cells, in which the gene expression pattern was consistent with the mutant phenotypes. Using double mutants, we investigated the genetic relationships between the mutations. The atabcg22 mutation further increased the water loss of srk2e/ost1 mutants, which were defective in ABA signalling in guard cells. Also, the atabcg22 mutation enhanced the phenotype of nced3 mutants, which were defective in ABA biosynthesis. Accordingly, the additive roles of AtABCG22 functions in ABA signalling and ABA biosynthesis are discussed.
Volume 67(5)
Pages 885-94
Published 2011-9
DOI 10.1111/j.1365-313X.2011.04641.x
PMID 21575091
MeSH ATP-Binding Cassette Transporters / genetics ATP-Binding Cassette Transporters / metabolism* Abscisic Acid / metabolism Arabidopsis / genetics Arabidopsis / physiology* Arabidopsis / ultrastructure Arabidopsis Proteins / genetics Arabidopsis Proteins / metabolism* Droughts Flowers / genetics Flowers / physiology Fruit / genetics Fruit / physiology Gene Expression Regulation, Plant / physiology Genotype Mutagenesis, Insertional Onions / genetics Onions / metabolism Onions / ultrastructure Phenotype Phylogeny Plant Growth Regulators / metabolism Plant Leaves / genetics Plant Leaves / metabolism Plant Leaves / physiology Plant Roots / genetics Plant Roots / physiology Plant Stems / genetics Plant Stems / physiology Plant Stomata / genetics Plant Stomata / physiology Plant Transpiration / genetics* Plants, Genetically Modified / genetics Plants, Genetically Modified / physiology Plants, Genetically Modified / ultrastructure Seedlings / genetics Seedlings / physiology Signal Transduction Water / metabolism*
IF 5.775
Times Cited 50
WOS Category PLANT SCIENCES
Resource
Arabidopsis / Cultured plant cells, genes pda04786