論文 - 詳細
| RRC ID | 1535 |
|---|---|
| 著者 | Pang Z, Otaka K, Maoka T, Hidaka K, Ishijima S, Oda M, Ohnishi M. |
| タイトル | Structure of beta-glucan oligomer from laminarin and its effect on human monocytes to inhibit the proliferation of U937 cells. |
| ジャーナル | Biosci Biotechnol Biochem |
| Abstract |
We analyzed the human monocyte-stimulating ability of laminarin from Eisenia bicyclis, lichenan from Cetraria islandica, and their oligomers depolymerized with endo-1,3-beta-glucanase from Arthrobacter sp. The respective beta-glucan oligomers with different degrees of polymerization (DP) were fractionated from hydrolytic products of laminarin and lichenan using gel-filtration chromatography. The monocyte-conditioned medium pre-cultured in the presence of a fraction of beta-glucan oligomer (DP>/=8) from laminarin exhibited inhibitory activity against the proliferation of human myeloid leukemia U937 cells, while those pre-cultured with other beta-glucan oligomers and the original laminarin and lichenan showed little or no activity. NMR analysis indicated that the beta-glucan oligomer (DP>/=8) has an average DP value of 13, and its ratio of beta-1,3- to beta-1,6-linkages in glucopyranose units was estimated to be 1.3:1. These results indicate that the beta-1,3-glucan oligomer with a higher content of beta-1,6-linkage stimulates monocytes to inhibit the proliferation of U937 cells. |
| 巻・号 | 69(3) |
| ページ | 553-8 |
| 公開日 | 2005-3-1 |
| DOI | 10.1271/bbb.69.553 |
| PII | JST.JSTAGE/bbb/69.553 |
| PMID | 15784984 |
| MeSH | Biopolymers / chemistry* Biopolymers / pharmacology Cell Division / drug effects* Glucans Humans Molecular Structure Monocytes / cytology Monocytes / drug effects* Nuclear Magnetic Resonance, Biomolecular Polysaccharides / chemistry* U937 Cells beta-Glucans / chemistry* beta-Glucans / pharmacology |
| IF | 1.516 |
| 引用数 | 64 |
| WOS 分野 | CHEMISTRY, APPLIED FOOD SCIENCE & TECHNOLOGY BIOTECHNOLOGY & APPLIED MICROBIOLOGY BIOCHEMISTRY & MOLECULAR BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 8 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | U-937 DE-4(RCB0435) |