| RRC ID |
15350
|
| Author |
Takahama K, Tomita J, Ueno T, Yamazaki M, Kume S, Kume K.
|
| Title |
Pan-neuronal knockdown of the c-Jun N-terminal Kinase (JNK) results in a reduction in sleep and longevity in Drosophila.
|
| Journal |
Biochem Biophys Res Commun
|
| Abstract |
Sleep is a unique behavioral state that is conserved between species, and sleep regulation is closely associated to metabolism and aging. The fruit fly, Drosophila melanogaster has been used to study the molecular mechanism underlying these physiological processes. Here we show that the c-Jun N-terminal Kinase (JNK) gene, known as basket (bsk) in Drosophila, functions in neurons to regulate both sleep and longevity in Drosophila. Pan-neuronal knockdown of JNK mRNA expression by RNA interference resulted in a decrease in both sleep and longevity. A heterozygous knockout of JNK showed similar effects, indicating the molecular specificity. The JNK knockdown showed a normal arousal threshold and sleep rebound, suggesting that the basic sleep mechanism was not affected. JNK is known to be involved in the insulin pathway, which regulates metabolism and longevity. A JNK knockdown in insulin-producing neurons in the pars intercerebralis had slight effects on sleep. However, knocking down JNK in the mushroom body had a significant effect on sleep. These data suggest a unique sleep regulating pathway for JNK.
|
| Volume |
417(2)
|
| Pages |
807-11
|
| Published |
2012-1-13
|
| DOI |
10.1016/j.bbrc.2011.12.040
|
| PII |
S0006-291X(11)02249-2
|
| PMID |
22197814
|
| MeSH |
Animals
Drosophila melanogaster / enzymology
Drosophila melanogaster / genetics
Drosophila melanogaster / physiology*
Gene Knockdown Techniques
JNK Mitogen-Activated Protein Kinases / genetics*
Longevity / genetics*
Neurons / enzymology*
RNA Interference
Sleep / genetics*
|
| IF |
2.985
|
| Times Cited |
16
|
|
WOS Category
|
BIOPHYSICS
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| Resource |
| Drosophila |
Stock(s) of DGRC Kyoto |
