RRC ID 15417
Author Nishimoto M, Kitaoka M.
Title Identification of the putative proton donor residue of lacto-N-biose phosphorylase (EC 2.4.1.211).
Journal Biosci. Biotechnol. Biochem.
Abstract Two lacto-N-biose phosphorylase (LNBP) isozyme genes were cloned from Bifidobacterium bifidum JCM1254. Alignment of the amino acid sequences of LNBP and its homologs identified 24 completely conserved acidic amino acid residues. All single mutants of Bifidobacterium longum LNBP at residues other than D313N retained considerable activity, suggesting that Asp313 is the putative proton donor residue in LNBP.
Volume 71(6)
Pages 1587-91
Published 2007-6
DOI 10.1271/bbb.70064
PII JST.JSTAGE/bbb/70064
PMID 17587697
MeSH Amino Acids Bifidobacterium / enzymology* Bifidobacterium / genetics Cloning, Molecular Conserved Sequence Isoenzymes Molecular Sequence Data Mutation Phosphorylases / genetics* Phosphorylases / metabolism Protons
IF 1.255
Times Cited 26
WOS Category FOOD SCIENCE & TECHNOLOGY CHEMISTRY, APPLIED BIOTECHNOLOGY & APPLIED MICROBIOLOGY BIOCHEMISTRY & MOLECULAR BIOLOGY
Resource
General Microbes JCM 1254