論文 - 詳細
| RRC ID | 15417 |
|---|---|
| 著者 | Nishimoto M, Kitaoka M. |
| タイトル | Identification of the putative proton donor residue of lacto-N-biose phosphorylase (EC 2.4.1.211). |
| ジャーナル | Biosci Biotechnol Biochem |
| Abstract |
Two lacto-N-biose phosphorylase (LNBP) isozyme genes were cloned from Bifidobacterium bifidum JCM1254. Alignment of the amino acid sequences of LNBP and its homologs identified 24 completely conserved acidic amino acid residues. All single mutants of Bifidobacterium longum LNBP at residues other than D313N retained considerable activity, suggesting that Asp313 is the putative proton donor residue in LNBP. |
| 巻・号 | 71(6) |
| ページ | 1587-91 |
| 公開日 | 2007-6-1 |
| DOI | 10.1271/bbb.70064 |
| PII | JST.JSTAGE/bbb/70064 |
| PMID | 17587697 |
| MeSH | Amino Acids Bifidobacterium / enzymology* Bifidobacterium / genetics Cloning, Molecular Conserved Sequence Isoenzymes Molecular Sequence Data Mutation Phosphorylases / genetics* Phosphorylases / metabolism Protons |
| IF | 1.516 |
| 引用数 | 32 |
| WOS 分野 | FOOD SCIENCE & TECHNOLOGY CHEMISTRY, APPLIED BIOTECHNOLOGY & APPLIED MICROBIOLOGY BIOCHEMISTRY & MOLECULAR BIOLOGY |
| リソース情報 | |
| 一般微生物 | JCM 1254 |