| RRC ID |
15419
|
| Author |
Iwatani S, Zendo T, Yoneyama F, Nakayama J, Sonomoto K.
|
| Title |
Characterization and structure analysis of a novel bacteriocin, lacticin Z, produced by Lactococcus lactis QU 14.
|
| Journal |
Biosci Biotechnol Biochem
|
| Abstract |
A novel bacteriocin, lacticin Z, produced by Lactococcus lactis QU 14 isolated from a horse's intestinal tract was identified. Lacticin Z was purified through a three step procedure comprised of hydrophobic-interaction, cation-exchange chromatography, and reverse-phase HPLC. ESI-TOF MS determined the molecular mass of lacticin Z to be 5,968.9 Da. The primary structure of lacticin Z was found to consist of 53 amino acid residues without any leader sequence or signal peptide. Lacticin Z showed homology to lacticin Q from L. lactis QU 5, aureocin A53 from Staphylococcus aureus A53, and mutacin BHT-B from Streptococcus rattus strain BHT. It exhibited a nanomolar range of MICs against various Gram-positive bacteria, and the activity was completely stable up to 100 degrees C. Unlike many of other LAB bacteriocins, the stability of lacticin Z was emphasized under alkaline conditions rather than acidic conditions. All the results indicated that lacticin Z belongs to a novel type of bacteriocin.
|
| Volume |
71(8)
|
| Pages |
1984-92
|
| Published |
2007-8-1
|
| DOI |
10.1271/bbb.70169
|
| PII |
JST.JSTAGE/bbb/70169
|
| PMID |
17690480
|
| MeSH |
Amino Acid Sequence
Animals
Bacterial Proteins
Bacteriocins / chemistry
Bacteriocins / isolation & purification
Bacteriocins / pharmacology*
Chromatography
Gram-Positive Bacteria / drug effects
Horses
Lactococcus lactis / chemistry*
Microbial Sensitivity Tests
Sequence Homology, Amino Acid
Spectrometry, Mass, Electrospray Ionization
|
| IF |
1.516
|
| Times Cited |
38
|
|
WOS Category
|
FOOD SCIENCE & TECHNOLOGY
CHEMISTRY, APPLIED
BIOTECHNOLOGY & APPLIED MICROBIOLOGY
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| Resource |
| General Microbes |
JCM 1059
JCM 1070
JCM 1132
JCM 1134
JCM 1157
JCM 1164
JCM 1465
JCM 2504
JCM 2257
JCM 5705
JCM 5706
JCM 5707
JCM 5802
JCM 5885
JCM 5887
JCM 6124
JCM 7638
JCM 8797 |
