RRC ID 15420
著者 Nishimoto M, Kitaoka M.
タイトル Practical preparation of lacto-N-biose I, a candidate for the bifidus factor in human milk.
ジャーナル Biosci Biotechnol Biochem
Abstract A one-pot enzymatic reaction to produce lacto-N-biose I (LNB), which is supposed to represent the bifidus factor in human milk oligosaccharides, was demonstrated. Approximately 500 mM of LNB was generated in 10-liter of reaction mixture initially containing 660 mM of sucrose and 600 mM of GlcNAc by the concurrent actions of four enzymes, sucrose phosphorylase, UDP-glucose-hexose-1-phospate uridylyltransferase, UDP-glucose 4-epimerase, and lacto-N-biose phosphorylase, in the presence of UDP-Glc and phosphate, indicating a reaction yield of 83%. LNB was isolated from the mixture by crystallization after yeast treatment. Finally, 1.4 kg of LNB of 99.6% purity was recovered after recrystallization.
巻・号 71(8)
ページ 2101-4
公開日 2007-8-1
DOI 10.1271/bbb.70320
PII JST.JSTAGE/bbb/70320
PMID 17690443
MeSH Acetylglucosamine / analogs & derivatives* Acetylglucosamine / chemical synthesis Crystallization Enzymes / metabolism Female Humans Milk, Human / chemistry* Molecular Sequence Data Oligosaccharides
IF 1.516
引用数 98
WOS 分野 FOOD SCIENCE & TECHNOLOGY CHEMISTRY, APPLIED BIOTECHNOLOGY & APPLIED MICROBIOLOGY BIOCHEMISTRY & MOLECULAR BIOLOGY
リソース情報
一般微生物 JCM 1217