RRC ID 15431
Author Kawano K, Ebisawa M, Hase K, Fukuda S, Hijikata A, Kawano S, Date Y, Tsuneda S, Itoh K, Ohno H.
Title Psg18 is specifically expressed in follicle-associated epithelium.
Journal Cell Struct. Funct.
Abstract Pregnancy-specific glycoproteins (Psgs) secreted by the placenta regulate the immune system to ensure the survival of the fetal allograft by inducing IL-10, an anti-inflammatory cytokine. However, it is unknown whether Psgs are involved in more general aspects of immune response other than maternal immunity. Here, we report that Psg18 is highly expressed in the follicle-associated epithelium (FAE) overlaying Peyer's patches (PPs). Bioinformatics analysis with Reference Database for Immune Cells (RefDIC) as well as RT-PCR data demonstrated that Psg18 is exclusively expressed in FAE in adult mice, in contrast to other Psg family members that are either not expressed or only slightly expressed in FAE. Psg18 expression was observed in FAE of germ-free-conditioned mice, and was slightly upregulated after bacterial inoculation. In situ hybridization analysis revealed that Psg18 is widely expressed throughout FAE. Furthermore, Psg18 protein is deposited on the extracellular matrix in the subepithelial dome beneath FAE, where antigen-presenting cells accumulate. These results suggest that Psg18 is an FAE-specific marker protein that could promote interplay between FAE and immune cells in mucosa-associated lymphoid tissues.
Volume 32(2)
Pages 115-26
Published 2007
PII JST.JSTAGE/csf/07014
PMID 17984568
MeSH Animals Computational Biology Epithelial Cells / immunology Epithelial Cells / metabolism* Extracellular Matrix / genetics Female Gene Expression Profiling* Humans In Situ Hybridization Male Mice Oligonucleotide Array Sequence Analysis Organ Specificity Ovarian Follicle / cytology Ovarian Follicle / metabolism* Peyer's Patches / cytology Pregnancy Pregnancy Proteins / genetics Pregnancy Proteins / immunology Pregnancy Proteins / metabolism* Protein Transport RNA Transport RNA, Messenger / genetics RNA, Messenger / metabolism Reverse Transcriptase Polymerase Chain Reaction Up-Regulation
IF 2.391
Times Cited 8
General Microbes JCM 1254