RRC ID 15444
Author Tada S, Katakura Y, Ninomiya K, Shioya S.
Title Fed-batch coculture of Lactobacillus kefiranofaciens with Saccharomyces cerevisiae for effective production of kefiran.
Journal J. Biosci. Bioeng.
Abstract In a batch coculture of kefiran-producing lactic acid bacteria Lactobacillus kefiranofaciens and lactate-assimilating yeast Saccharomyces cerevisiae, lactate accumulation in the medium was observed, which inhibited kefiran production. To enhance kefiran productivity by preventing lactate accumulation, we conducted lactose-feeding batch operation with feedforward/feedback control during the coculture, so that the lactate production rate of L. kefiranofaciens was balanced with the lactate consumption rate of S. cerevisiae. The lactate concentration was maintained at less than 6 g l(-1) throughout the fed-batch coculture using a 5 l jar fermentor, although the concentration reached 33 g l(-1) in the batch coculture. Kefiran production was increased to 6.3 g in 102 h in the fed-batch coculture, whereas 4.5 g kefiran was produced in 97 h in the batch coculture. The kefiran yield on lactose basis was increased up to 0.033 g g(-1) in the fed-batch coculture, whereas that in the batch coculture was 0.027 g g(-1).
Volume 103(6)
Pages 557-62
Published 2007-6
DOI 10.1263/jbb.103.557
PII S1389-1723(07)70104-4
PMID 17630128
MeSH Biotechnology Coculture Techniques / methods* Feedback Fermentation Kinetics Lactic Acid / metabolism Lactobacillus / metabolism* Models, Biological Polysaccharides / biosynthesis* Saccharomyces cerevisiae / metabolism*
IF 2.032
Times Cited 11
General Microbes JCM 6985