Reference - Detail
| RRC ID | 15445 |
|---|---|
| Author | Povelainen M, Miasnikov AN. |
| Title | Production of xylitol by metabolically engineered strains of Bacillus subtilis. |
| Journal | J Biotechnol |
| Abstract |
Xylitol-phosphate dehydrogenase (XPDH) genes from several Gram-positive bacteria were isolated and expressed in Bacillus subtilis. The substrate specificities of the recombinant XPDH enzymes were compared and it was found that the XPDH enzymes of Lactobacillus rhamnosus and Clostridium difficile had the highest selectivity towards D-xylulose 5-phosphate. Expression of these two XPDH enzymes in D-ribulose and D-xylulose producing B. subtilis strain resulted in strains of B. subtilis capable of converting D-glucose into xylitol at around 23% yield. |
| Volume | 128(1) |
| Pages | 24-31 |
| Published | 2007-1-30 |
| DOI | 10.1016/j.jbiotec.2006.09.008 |
| PII | S0168-1656(06)00781-4 |
| PMID | 17079043 |
| MeSH | Bacillus subtilis / enzymology Bacillus subtilis / genetics Bacillus subtilis / metabolism* Bacterial Proteins / metabolism Clostridioides difficile / enzymology Clostridioides difficile / genetics Fermentation Genetic Engineering Glucose / metabolism Lactobacillus rhamnosus / enzymology Lactobacillus rhamnosus / genetics Recombinant Proteins / metabolism* Ribulosephosphates / metabolism Sugar Alcohol Dehydrogenases / genetics* Xylitol / metabolism* Xylose / metabolism |
| IF | 3.503 |
| Times Cited | 26 |
| WOS Category | BIOTECHNOLOGY & APPLIED MICROBIOLOGY |
| Resource | |
| General Microbes | JCM 9153 |