RRC ID 15477
著者 Kageyama Y, Takaki Y, Shimamura S, Nishi S, Nogi Y, Uchimura K, Kobayashi T, Hitomi J, Ozaki K, Kawai S, Ito S, Horikoshi K.
タイトル Intragenomic diversity of the V1 regions of 16S rRNA genes in high-alkaline protease-producing Bacillus clausii spp.
ジャーナル Extremophiles
Abstract Alkaliphilic Bacillus sp. strain KSM-K16, which produces high-alkaline M-protease, was characterized phenotypically, biochemically and genetically. This strain was identified as Bacillus clausii based on the results of taxonomic studies, including sequencing of the 16S rRNA gene and DNA-DNA hybridization. Seven rRNA operons in the genome were identified by pulsed-field gel electrophoresis. Sequencing of cloned 16S rRNA genes revealed two distinct types of variable region V1. Moreover, some cloned 16S rRNA genes in some of the reference strains of B. clausii had a V1 region of yet another type. The B. clausii strains could clearly be divided into at least two subgroups based on the frequencies of the types of cloned V1 sequence. Bacillus sp. strain KSM-K16 was found to be in a different phylogenetic position from other high-alkaline protease-producing strains of B. clausii.
巻・号 11(4)
ページ 597-603
公開日 2007-7-1
DOI 10.1007/s00792-007-0074-1
PMID 17429572
MeSH Bacillus / classification* Bacillus / enzymology Bacillus / genetics Base Sequence Cloning, Molecular DNA, Bacterial / analysis* DNA, Ribosomal / analysis* Electrophoresis, Gel, Pulsed-Field Genetic Variation* Genotype Molecular Sequence Data Nucleic Acid Hybridization Operon Phenotype Phylogeny RNA, Ribosomal, 16S* Ribotyping* Sequence Analysis, DNA Serine Endopeptidases / metabolism* Subtilisins / metabolism*
IF 2.462
引用数 14
WOS 分野 MICROBIOLOGY BIOCHEMISTRY & MOLECULAR BIOLOGY
リソース情報
一般微生物 JCM 9158 JCM 9160