| RRC ID |
15482
|
| 著者 |
Li Y, Canchaya C, Fang F, Raftis E, Ryan KA, van Pijkeren JP, van Sinderen D, O'Toole PW.
|
| タイトル |
Distribution of megaplasmids in Lactobacillus salivarius and other lactobacilli.
|
| ジャーナル |
J Bacteriol
|
| Abstract |
The genome of Lactobacillus salivarius UCC118 includes a 242-kb megaplasmid, pMP118. We now show that 33 strains of L. salivarius isolated from humans and animals all harbor a megaplasmid, which hybridized with the repA and repE replication origin probes of pMP118. Linear megaplasmids that did not hybridize with the pMP118 repA probe were also found in some strains of L. salivarius, showing for the first time that a lactic acid bacterium has multiple megaplasmids. Phylogenetic analysis of the repE and groEL sequences of 28 L. salivarius strains suggested similar evolutionary paths for the chromosome and megaplasmid. Although the replication origin of circular megaplasmids in L. salivarius was highly conserved, genotypic and phenotypic comparisons revealed significant variation between megaplasmid-encoded traits. Furthermore, megaplasmids of sizes ranging from 120 kb to 490 kb were present in seven strains belonging to six other Lactobacillus species from among 91 strains and 47 species tested. The discovery of the widespread presence of megaplasmids in L. salivarius, and restricted carriage by other Lactobacillus species, provides an opportunity to study the contribution of large extrachromosomal replicons to the biology of Lactobacillus.
|
| 巻・号 |
189(17)
|
| ページ |
6128-39
|
| 公開日 |
2007-9-1
|
| DOI |
10.1128/JB.00447-07
|
| PII |
JB.00447-07
|
| PMID |
17586640
|
| PMC |
PMC1951925
|
| MeSH |
Aged
Animals
Bacterial Proteins / genetics
Chaperonin 60 / genetics
Conserved Sequence
DNA, Bacterial / chemistry
DNA, Bacterial / genetics
Female
Genes, Bacterial / genetics
Genetic Variation
Humans
Infant
Lactobacillus / genetics*
Lactobacillus / isolation & purification
Male
Middle Aged
Molecular Sequence Data
Nucleic Acid Hybridization
Phylogeny
Plasmids / chemistry
Plasmids / genetics*
Replication Origin / genetics
Replication Protein A / genetics
Sequence Analysis, DNA
Sequence Homology
|
| IF |
3.006
|
| 引用数 |
42
|
|
WOS 分野
|
MICROBIOLOGY
|
| リソース情報 |
| 一般微生物 |
JCM 1040
JCM 1042
JCM 1044
JCM 1045
JCM 1046
JCM 1047
JCM 1230 |
