論文 - 詳細
| RRC ID | 15487 |
|---|---|
| 著者 | Tam KJ, Watson CT, Massah S, Kolybaba AM, Breden F, Prefontaine GG, Beischlag TV. |
| タイトル | Regulatory function of conserved sequences upstream of the long-wave sensitive opsin genes in teleost fishes. |
| ジャーナル | Vision Res |
| Abstract |
Vertebrate opsin genes often occur in sets of tandem duplicates, and their expression varies developmentally and in response to environmental cues. We previously identified two highly conserved regions upstream of the long-wave sensitive opsin (LWS) gene cluster in teleosts. This region has since been shown in zebrafish to drive expression of LWS genes in vivo. In order to further investigate how elements in this region control opsin gene expression, we tested constructs encompassing the highly conserved regions and the less conserved portions upstream of the coding sequences in a promoter-less luciferase expression system. A ∼4500 bp construct of the upstream region, including the highly-conserved regions Reg I and Reg II, increased expression 100-fold, and successive 5' deletions reduced expression relative to the full 4.5 Kb region. Gene expression was highest when the transcription factor RORα was co-transfected with the proposed regulatory regions. Because these regions were tested in a promoter-less expression system, they include elements able to initiate and drive transcription. Teleosts exhibit complex color-mediated adaptive behavior and their adaptive significance has been well documented in several species. Therefore these upstream regions of LWS represent a model system for understanding the molecular basis of adaptive variation in gene regulation of color vision. |
| 巻・号 | 51(21-22) |
| ページ | 2295-303 |
| 公開日 | 2011-11-1 |
| DOI | 10.1016/j.visres.2011.09.010 |
| PII | S0042-6989(11)00340-3 |
| PMID | 21971525 |
| MeSH | Animals Color Vision / genetics* Color Vision / physiology Cone Opsins / genetics* Cone Opsins / metabolism Conserved Sequence / physiology* Enhancer Elements, Genetic Fishes / genetics* Gene Expression Regulation* Genes, Reporter Luciferases Protein Binding / genetics Receptors, Retinoic Acid / metabolism* Retinal Cone Photoreceptor Cells Retinoic Acid Receptor alpha Transcription Factors / metabolism |
| IF | 2.61 |
| 引用数 | 8 |
| WOS 分野 | OPHTHALMOLOGY NEUROSCIENCES |
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| 各媒体での言及数の合計 | 2 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
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| メダカ | database |