RRC ID 15558
著者 Jones TA, Metzstein MM.
タイトル A novel function for the PAR complex in subcellular morphogenesis of tracheal terminal cells in Drosophila melanogaster.
ジャーナル Genetics
Abstract The processes that generate cellular morphology are not well understood. To investigate this problem, we use Drosophila melanogaster tracheal terminal cells, which undergo two distinct morphogenetic processes: subcellular branching morphogenesis and subcellular apical lumen formation. Here we show these processes are regulated by components of the PAR-polarity complex. This complex, composed of the proteins Par-6, Bazooka (Par-3), aPKC, and Cdc42, is best known for roles in asymmetric cell division and apical/basal polarity. We find Par-6, Bazooka, and aPKC, as well as known interactions between them, are required for subcellular branch initiation, but not for branch outgrowth. By analysis of single and double mutants, and isolation of two novel alleles of Par-6, one of which specifically truncates the Par-6 PDZ domain, we conclude that dynamic interactions between apical PAR-complex members control the branching pattern of terminal cells. These data suggest that canonical apical PAR-complex activity is required for subcellular branching morphogenesis. In addition, we find the PAR proteins are downstream of the FGF pathway that controls terminal cell branching. In contrast, we find that while Par-6 and aPKC are both required for subcellular lumen formation, neither Bazooka nor a direct interaction between Par-6 and aPKC is needed for this process. Thus a novel, noncanonical role for the polarity proteins Par-6 and aPKC is used in formation of this subcellular apical compartment. Our results demonstrate that proteins from the PAR complex can be deployed independently within a single cell to control two different morphogenetic processes.
巻・号 189(1)
ページ 153-64
公開日 2011-9-1
DOI 10.1534/genetics.111.130351
PII genetics.111.130351
PMID 21750259
PMC PMC3176136
MeSH Animals Drosophila Proteins / metabolism* Drosophila melanogaster / cytology* Drosophila melanogaster / embryology Drosophila melanogaster / metabolism Fibroblast Growth Factors / metabolism Intracellular Signaling Peptides and Proteins / metabolism Intracellular Space / metabolism Morphogenesis* PDZ Domains Protein Kinase C / metabolism* Protein Transport Signal Transduction Trachea / cytology
IF 4.015
引用数 8
WOS 分野 GENETICS & HEREDITY
リソース情報
ショウジョウバエ 5055R-1 5055R-2