論文 - 詳細
| RRC ID | 15605 |
|---|---|
| 著者 | Weelink SA, Tan NC, ten Broeke H, van den Kieboom C, van Doesburg W, Langenhoff AA, Gerritse J, Junca H, Stams AJ. |
| タイトル | Isolation and characterization of Alicycliphilus denitrificans strain BC, which grows on benzene with chlorate as the electron acceptor. |
| ジャーナル | Appl Environ Microbiol |
| Abstract |
A bacterium, strain BC, was isolated from a benzene-degrading chlorate-reducing enrichment culture. Strain BC degrades benzene in conjunction with chlorate reduction. Cells of strain BC are short rods that are 0.6 microm wide and 1 to 2 microm long, are motile, and stain gram negative. Strain BC grows on benzene and some other aromatic compounds with oxygen or in the absence of oxygen with chlorate as the electron acceptor. Strain BC is a denitrifying bacterium, but it is not able to grow on benzene with nitrate. The closest cultured relative is Alicycliphilus denitrificans type strain K601, a cyclohexanol-degrading nitrate-reducing betaproteobacterium. Chlorate reductase (0.4 U/mg protein) and chlorite dismutase (5.7 U/mg protein) activities in cell extracts of strain BC were determined. Gene sequences encoding a known chlorite dismutase (cld) were not detected in strain BC by using the PCR primers described in previous studies. As physiological and biochemical data indicated that there was oxygenation of benzene during growth with chlorate, a strategy was developed to detect genes encoding monooxygenase and dioxygenase enzymes potentially involved in benzene degradation in strain BC. Using primer sets designed to amplify members of distinct evolutionary branches in the catabolic families involved in benzene biodegradation, two oxygenase genes putatively encoding the enzymes performing the initial successive monooxygenations (BC-BMOa) and the cleavage of catechol (BC-C23O) were detected. Our findings suggest that oxygen formed by dismutation of chlorite can be used to attack organic molecules by means of oxygenases, as exemplified with benzene. Thus, aerobic pathways can be employed under conditions in which no external oxygen is supplied. |
| 巻・号 | 74(21) |
| ページ | 6672-81 |
| 公開日 | 2008-11-1 |
| DOI | 10.1128/AEM.00835-08 |
| PII | AEM.00835-08 |
| PMID | 18791031 |
| PMC | PMC2576721 |
| MeSH | Bacterial Proteins / genetics Bacterial Proteins / metabolism Benzene / metabolism* Chlorates / metabolism* Comamonadaceae / classification* Comamonadaceae / genetics Comamonadaceae / isolation & purification* Comamonadaceae / metabolism DNA, Bacterial / chemistry DNA, Bacterial / genetics DNA, Ribosomal / chemistry DNA, Ribosomal / genetics Dioxygenases / genetics Dioxygenases / metabolism Genes, rRNA Locomotion Mixed Function Oxygenases / genetics Mixed Function Oxygenases / metabolism Molecular Sequence Data Nitrates / metabolism Nitrites / metabolism Nitrogen / metabolism Oxidoreductases / genetics Oxidoreductases / metabolism Oxygen / metabolism Phylogeny Polymerase Chain Reaction / methods RNA, Bacterial / genetics RNA, Ribosomal, 16S / genetics Sequence Analysis, DNA Sequence Homology, Nucleic Acid |
| IF | 4.016 |
| 引用数 | 63 |
| WOS 分野 | BIOTECHNOLOGY & APPLIED MICROBIOLOGY MICROBIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Wikipedia |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 一般微生物 | JCM 14587 |