論文 - 詳細
| RRC ID | 15609 |
|---|---|
| 著者 | Park SC, Pham BP, Van Duyet L, Jia B, Lee S, Yu R, Han SW, Yang JK, Hahm KS, Cheong GW. |
| タイトル | Structural and functional characterization of osmotically inducible protein C (OsmC) from Thermococcus kodakaraensis KOD1. |
| ジャーナル | Biochim Biophys Acta |
| Abstract |
Osmotically inducible protein C (OsmC) is involved in the cellular defense mechanism against oxidative stress caused by exposure to hyperoxides or elevated osmolarity. OsmC was identified by two-dimensional electrophoresis (2DE) analysis as a protein that is overexpressed in response to osmotic stress, but not under heat and oxidative stress. Here, an OsmC gene from T. kodakaraensis KOD1 was cloned and expressed in Escherichia coli. TkOsmC showed a homotetrameric structure based on gel filtration and electron microscopic analyses. TkOsmC has a significant peroxidase activity toward both organic and inorganic peroxides in high, but not in low temperature. |
| 巻・号 | 1784(5) |
| ページ | 783-8 |
| 公開日 | 2008-5-1 |
| DOI | 10.1016/j.bbapap.2008.02.002 |
| PII | S1570-9639(08)00046-0 |
| PMID | 18319068 |
| MeSH | Amino Acid Sequence Archaeal Proteins / chemistry* Archaeal Proteins / metabolism* Archaeal Proteins / ultrastructure Chromatography, Gel Cross-Linking Reagents / pharmacology Hot Temperature Hydrogen Peroxide / metabolism Molecular Sequence Data Osmotic Pressure / drug effects Oxidative Stress / drug effects Peroxidase / metabolism Protein Structure, Quaternary Sequence Homology, Amino Acid Thermococcus / drug effects Thermococcus / enzymology Thermococcus / metabolism* tert-Butylhydroperoxide / metabolism |
| IF | 3.411 |
| 引用数 | 21 |
| WOS 分野 | BIOPHYSICS BIOCHEMISTRY & MOLECULAR BIOLOGY |
| リソース情報 | |
| 一般微生物 | JCM 12380 |