RRC ID 15609
Author Park SC, Pham BP, Van Duyet L, Jia B, Lee S, Yu R, Han SW, Yang JK, Hahm KS, Cheong GW.
Title Structural and functional characterization of osmotically inducible protein C (OsmC) from Thermococcus kodakaraensis KOD1.
Journal Biochim. Biophys. Acta
Abstract Osmotically inducible protein C (OsmC) is involved in the cellular defense mechanism against oxidative stress caused by exposure to hyperoxides or elevated osmolarity. OsmC was identified by two-dimensional electrophoresis (2DE) analysis as a protein that is overexpressed in response to osmotic stress, but not under heat and oxidative stress. Here, an OsmC gene from T. kodakaraensis KOD1 was cloned and expressed in Escherichia coli. TkOsmC showed a homotetrameric structure based on gel filtration and electron microscopic analyses. TkOsmC has a significant peroxidase activity toward both organic and inorganic peroxides in high, but not in low temperature.
Volume 1784(5)
Pages 783-8
Published 2008-5
DOI 10.1016/j.bbapap.2008.02.002
PII S1570-9639(08)00046-0
PMID 18319068
MeSH Amino Acid Sequence Archaeal Proteins / chemistry* Archaeal Proteins / metabolism* Archaeal Proteins / ultrastructure Chromatography, Gel Cross-Linking Reagents / pharmacology Hot Temperature Hydrogen Peroxide / metabolism Molecular Sequence Data Osmotic Pressure / drug effects Oxidative Stress / drug effects Peroxidase / metabolism Protein Structure, Quaternary Sequence Homology, Amino Acid Thermococcus / drug effects Thermococcus / enzymology Thermococcus / metabolism* tert-Butylhydroperoxide / metabolism
IF 3.438
Times Cited 15
General Microbes JCM 12380