Reference - Detail
| RRC ID | 15615 |
|---|---|
| Author | Fukao M, Obita T, Yoneyama F, Kohda D, Zendo T, Nakayama J, Sonomoto K. |
| Title | Complete covalent structure of nisin Q, new natural nisin variant, containing post-translationally modified amino acids. |
| Journal | Biosci Biotechnol Biochem |
| Abstract |
The third member of the nisin variant, nisin Q, produced by Lactococcus lactis 61-14, is a ribosomally-synthesized antimicrobial peptide, the so-called lantibiotic containing post-translationally modified amino acids such as lanthionine and dehydroalanine. Here, we determined the complete covalent structure of nisin Q, consisting of 34 amino acids, by two-dimensional (1)H nuclear magnetic resonance (NMR) spectroscopy. Sequential assignment of nisin Q containing the unusual amino acids was performed by total correlation spectroscopy (TOCSY) and nuclear Overhauser enhancement spectroscopy (NOESY). The observed long range nuclear Overhauser effect (NOE) in nisin Q indicated assignment of all five sets of lanthionines that intramolecularly bridge residues 3-7, 8-11, 13-19, 23-26, and 25-28. Consequently, the covalent structure of nisin Q was determined to hold the same thioether linkage formation as the other two nisins, but to harbor the four amino acid substitutions, in contrast with nisin A. |
| Volume | 72(7) |
| Pages | 1750-5 |
| Published | 2008-7-1 |
| DOI | 10.1271/bbb.80066 |
| PII | JST.JSTAGE/bbb/80066 |
| PMID | 18603791 |
| MeSH | Alanine / analogs & derivatives Amino Acid Substitution Anti-Bacterial Agents Bacteriocins Magnetic Resonance Spectroscopy Molecular Structure Nisin / biosynthesis Nisin / chemistry* Protein Processing, Post-Translational* Sulfides |
| IF | 1.516 |
| Times Cited | 8 |
| WOS Category | FOOD SCIENCE & TECHNOLOGY CHEMISTRY, APPLIED BIOTECHNOLOGY & APPLIED MICROBIOLOGY BIOCHEMISTRY & MOLECULAR BIOLOGY |
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| The most frequently cited source | Patent(IFI CLAIMS) |
| Total number of mentions | 1 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| General Microbes | JCM 2257 |