| RRC ID |
15621
|
| Author |
Anggraeni AA, Sakka M, Kimura T, Ratanakhaokchai K, Kitaoka M, Sakka K.
|
| Title |
Characterization of Bacillus halodurans alpha-galactosidase Mel4A encoded by the mel4A gene (BH2228).
|
| Journal |
Biosci Biotechnol Biochem
|
| Abstract |
A family-4 alpha-galactosidase Mel4A of Bacillus halodurans was expressed in Escherichia coli and characterized. Recombinant enzyme rMel4A depended on NAD+, some divalent cations such as Mn2+, and reducing reagents such as dithiothreitol. rMel4A was active on small saccharides such as raffinose but not on highly polymerized galactomannan. Immunological analysis indicated that raffinose induced the production of Mel4A in B. halodurans.
|
| Volume |
72(9)
|
| Pages |
2459-62
|
| Published |
2008-9-1
|
| DOI |
10.1271/bbb.80242
|
| PII |
JST.JSTAGE/bbb/80242
|
| PMID |
18776668
|
| MeSH |
Bacillus / enzymology*
Bacillus / genetics
Bacillus / metabolism
Cations, Divalent / metabolism
Dithiothreitol / pharmacology
Dose-Response Relationship, Drug
Escherichia coli / genetics
Genes, Bacterial*
Manganese / metabolism
Melibiose / genetics*
NAD / metabolism
NAD / pharmacology
Raffinose / metabolism
Recombinant Proteins / isolation & purification
Recombinant Proteins / metabolism
alpha-Galactosidase / genetics*
alpha-Galactosidase / isolation & purification
alpha-Galactosidase / metabolism*
|
| IF |
1.516
|
| Times Cited |
9
|
|
WOS Category
|
FOOD SCIENCE & TECHNOLOGY
CHEMISTRY, APPLIED
BIOTECHNOLOGY & APPLIED MICROBIOLOGY
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| Resource |
| General Microbes |
JCM 9153 |
