Reference - Detail
| RRC ID | 15625 |
|---|---|
| Author | Fujii T, Takaya N. |
| Title | Denitrification by the fungus Fusarium oxysporum involves NADH-nitrate reductase. |
| Journal | Biosci Biotechnol Biochem |
| Abstract |
Fusarium oxysporum JCM11502 expresses a denitrifying (nitrate (NO(3)(-))-respiring) mechanism and can thrive under oxygen (O(2)) limitation. The fungus reduces NO(3)(-) to nitrite at the initial step of denitrification. In this study, we cloned the gene coding NADH-NO(3)(-) reductase (NADH-Nar) (niaD) from F. oxysporum JCM11502. The niaD gene complemented the defective NO(3)(-) assimilation by mutant strain M10, indicating that the fungus reduced NO(3)(-) through NADH-Nar activity and assimilated it like other fungi. We found that the transcription of niaD and the production of NADH-Nar activity were enhanced under O(2)-limited denitrifying conditions relative to aerobic conditions. Strain M10 produced less NADH-Nar activity and less denitrified product than the wild-type strain. Introducing niaD into the mutant also restored these defects, indicating that niaD is involved in denitrification. These results indicate that the fungus denitrified NO(3)(-) through NADH-Nar activity in addition to the ubiquinol-Nar mechanism. |
| Volume | 72(2) |
| Pages | 412-20 |
| Published | 2008-2-1 |
| DOI | 10.1271/bbb.70538 |
| PII | JST.JSTAGE/bbb/70538 |
| PMID | 18256499 |
| MeSH | Base Sequence Cloning, Molecular DNA Primers DNA, Complementary Fusarium / metabolism* Genes, Fungal Genetic Complementation Test Mutation Nitrate Reductase (NADH) / genetics Nitrate Reductase (NADH) / metabolism* Nitrates / metabolism* Plasmids Reverse Transcriptase Polymerase Chain Reaction |
| IF | 1.516 |
| Times Cited | 23 |
| WOS Category | FOOD SCIENCE & TECHNOLOGY CHEMISTRY, APPLIED BIOTECHNOLOGY & APPLIED MICROBIOLOGY BIOCHEMISTRY & MOLECULAR BIOLOGY |
| Resource | |
| General Microbes | JCM 11502 |