| RRC ID |
15626
|
| Author |
Sugimoto S, Saruwatari K, Higashi C, Tsuruno K, Matsumoto S, Nakayama J, Sonomoto K.
|
| Title |
In vivo and in vitro complementation study comparing the function of DnaK chaperone systems from halophilic lactic acid bacterium Tetragenococcus halophilus and Escherichia coli.
|
| Journal |
Biosci Biotechnol Biochem
|
| Abstract |
In this study, we characterized the DnaK chaperone system from Tetragenococcus halophilus, a halophilic lactic acid bacterium. An in vivo complementation test showed that under heat stress conditions, T. halophilus DnaK did not rescue the growth of the Escherichia coli dnaK deletion mutant, whereas T. halophilus DnaJ and GrpE complemented the corresponding mutations of E. coli. Purified T. halophilus DnaK showed intrinsic weak ATPase activity and holding chaperone activity in vitro, but T. halophilus DnaK did not cooperate with the purified DnaJ and GrpE from either T. halophilus or E. coli in ATP hydrolysis or luciferase-refolding reactions under the conditions tested. E. coli DnaK, however, cross-reacted with those from both bacteria. This difference in the cooperation with DnaJ and GrpE appears to result in an inability of T. halophilus DnaK to replace the in vivo function of the DnaK chaperone of E. coli.
|
| Volume |
72(3)
|
| Pages |
811-22
|
| Published |
2008-3-1
|
| DOI |
10.1271/bbb.70691
|
| PII |
JST.JSTAGE/bbb/70691
|
| PMID |
18323638
|
| MeSH |
Bacterial Proteins / genetics
Bacterial Proteins / physiology*
Escherichia coli Proteins / genetics
Escherichia coli Proteins / physiology*
Genetic Complementation Test
HSP40 Heat-Shock Proteins / genetics
HSP40 Heat-Shock Proteins / physiology
HSP70 Heat-Shock Proteins / genetics
HSP70 Heat-Shock Proteins / physiology
Heat Stress Disorders
Lactobacillus / chemistry*
Molecular Chaperones*
Mutation
|
| IF |
1.516
|
| Times Cited |
11
|
|
WOS Category
|
FOOD SCIENCE & TECHNOLOGY
CHEMISTRY, APPLIED
BIOTECHNOLOGY & APPLIED MICROBIOLOGY
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| Resource |
| General Microbes |
JCM 5888 |
