論文 - 詳細
| RRC ID | 15642 |
|---|---|
| 著者 | Hu CB, Zendo T, Nakayama J, Sonomoto K. |
| タイトル | Description of durancin TW-49M, a novel enterocin B-homologous bacteriocin in carrot-isolated Enterococcus durans QU 49. |
| ジャーナル | J Appl Microbiol |
| Abstract |
AIMS:To characterize the novel bacteriocin produced by Enterococcus durans. METHODS AND RESULTS:Enterococcus durans QU 49 was isolated from carrot and expressed bactericidal activity over 20-43 degrees C. Bacteriocins were purified to homogeneity using the three-step purification method, one of which, termed durancin TW-49M, was an enterocin B-homologous peptide with most identical residues occurring in the N-terminus. Durancin TW-49M was more tolerant in acidic than in alkali. DNA sequencing analysis revealed durancin TW-49M was translated as a prepeptide of the double-glycine type. Durancin TW-49M and enterocin B expressed similar antimicrobial spectra, in which no significant variation due to the diversity in their C-termini was observed. CONCLUSIONS:Durancin TW-49M, a novel nonpediocin-like class II bacteriocin, was characterized to the amino acid and genetic levels. The diverse C-terminal parts of durancin TW-49M and enterocin B were hardly to be suggested as the place determining the target cell specificity. SIGNIFICANCE AND IMPACT OF THE STUDY:This is the first and comprehensive study of a novel bacteriocin produced by Ent. durans. The high homology at the N-terminal halves between durancin TW-49M and enterocin B makes them suitable to study the structure-function relationship of bacteriocins and their immunity proteins. |
| 巻・号 | 105(3) |
| ページ | 681-90 |
| 公開日 | 2008-9-1 |
| DOI | 10.1111/j.1365-2672.2008.03798.x |
| PII | JAM3798 |
| PMID | 18397254 |
| MeSH | Amino Acid Sequence Anti-Bacterial Agents / isolation & purification* Anti-Bacterial Agents / metabolism Bacteriocins / genetics Bacteriocins / isolation & purification* Bacteriocins / metabolism Base Sequence Daucus carota / microbiology* Enterococcus / metabolism* Food Microbiology* Hot Temperature Hydrogen-Ion Concentration Microbial Sensitivity Tests Molecular Sequence Data Molecular Weight Sequence Analysis, DNA Sequence Homology |
| IF | 3.066 |
| 引用数 | 20 |
| WOS 分野 | BIOTECHNOLOGY & APPLIED MICROBIOLOGY MICROBIOLOGY |
| リソース情報 | |
| 一般微生物 | JCM 1059 JCM 1095 JCM 1123 JCM 1134 JCM 1157 JCM 1164 JCM 1465 JCM 2152 JCM 2257 JCM 2501 JCM 2504 JCM 5803 JCM 5804 JCM 5705 JCM 5706 JCM 5707 JCM 5803 JCM 5804 JCM 5885 JCM 5890 JCM 6124 JCM 8797 JCM 10707 JCM 11004 |