| RRC ID |
15643
|
| Author |
Jimenez N, Canals R, Saló MT, Vilches S, Merino S, Tomás JM.
|
| Title |
The Aeromonas hydrophila wb*O34 gene cluster: genetics and temperature regulation.
|
| Journal |
J Bacteriol
|
| Abstract |
The Aeromonas hydrophila wb*(O34) gene cluster of strain AH-3 (serotype O34) was cloned and sequenced. This cluster contains genes necessary for the production of O34-antigen lipopolysaccharide (LPS) in A. hydrophila. We determined, using either mutation or sequence homology, roles for the majority of genes in the cluster by using the chemical O34-antigen LPS structure obtained for strain AH-3. The O34-antigen LPS export system has been shown to be a Wzy-dependent pathway typical of heteropolysaccharide pathways. Furthermore, the production of A. hydrophila O34-antigen LPS in Escherichia coli K-12 strains is dependent on incorporation of the Gne enzyme (UDP-N-acetylgalactosamine 4-epimerase) necessary for the formation of UDP-galactosamine in these strains. By using rapid amplification of cDNA ends we were able to identify a transcription start site upstream of the terminal wzz gene, which showed differential transcription depending on the growth temperature of the strain. The Wzz protein is able to regulate the O34-antigen LPS chain length. The differential expression of this protein at different temperatures, which was substantially greater at 20 degrees C than at 37 degrees C, explains the previously observed differential production of O34-antigen LPS and its correlation with the virulence of A. hydrophila serotype O34 strains.
|
| Volume |
190(12)
|
| Pages |
4198-209
|
| Published |
2008-6-1
|
| DOI |
10.1128/JB.00153-08
|
| PII |
JB.00153-08
|
| PMID |
18408022
|
| PMC |
PMC2446748
|
| MeSH |
Aeromonas hydrophila / genetics*
Aeromonas hydrophila / metabolism
Bacterial Proteins / genetics
Bacterial Proteins / metabolism
Blotting, Southern
Carbohydrate Epimerases / genetics
Carbohydrate Epimerases / metabolism
Carbohydrate Sequence
Gene Expression Regulation, Bacterial
Models, Genetic
Molecular Sequence Data
Multigene Family*
O Antigens / chemistry
O Antigens / genetics*
O Antigens / metabolism
Reverse Transcriptase Polymerase Chain Reaction
Sequence Analysis, DNA
Temperature
Transcription, Genetic
|
| IF |
3.006
|
| Times Cited |
19
|
|
WOS Category
|
MICROBIOLOGY
|
| Resource |
| General Microbes |
JCM 3980 |
