論文 - 詳細
| RRC ID | 15643 |
|---|---|
| 著者 | Jimenez N, Canals R, Saló MT, Vilches S, Merino S, Tomás JM. |
| タイトル | The Aeromonas hydrophila wb*O34 gene cluster: genetics and temperature regulation. |
| ジャーナル | J Bacteriol |
| Abstract |
The Aeromonas hydrophila wb*(O34) gene cluster of strain AH-3 (serotype O34) was cloned and sequenced. This cluster contains genes necessary for the production of O34-antigen lipopolysaccharide (LPS) in A. hydrophila. We determined, using either mutation or sequence homology, roles for the majority of genes in the cluster by using the chemical O34-antigen LPS structure obtained for strain AH-3. The O34-antigen LPS export system has been shown to be a Wzy-dependent pathway typical of heteropolysaccharide pathways. Furthermore, the production of A. hydrophila O34-antigen LPS in Escherichia coli K-12 strains is dependent on incorporation of the Gne enzyme (UDP-N-acetylgalactosamine 4-epimerase) necessary for the formation of UDP-galactosamine in these strains. By using rapid amplification of cDNA ends we were able to identify a transcription start site upstream of the terminal wzz gene, which showed differential transcription depending on the growth temperature of the strain. The Wzz protein is able to regulate the O34-antigen LPS chain length. The differential expression of this protein at different temperatures, which was substantially greater at 20 degrees C than at 37 degrees C, explains the previously observed differential production of O34-antigen LPS and its correlation with the virulence of A. hydrophila serotype O34 strains. |
| 巻・号 | 190(12) |
| ページ | 4198-209 |
| 公開日 | 2008-6-1 |
| DOI | 10.1128/JB.00153-08 |
| PII | JB.00153-08 |
| PMID | 18408022 |
| PMC | PMC2446748 |
| MeSH | Aeromonas hydrophila / genetics* Aeromonas hydrophila / metabolism Bacterial Proteins / genetics Bacterial Proteins / metabolism Blotting, Southern Carbohydrate Epimerases / genetics Carbohydrate Epimerases / metabolism Carbohydrate Sequence Gene Expression Regulation, Bacterial Models, Genetic Molecular Sequence Data Multigene Family* O Antigens / chemistry O Antigens / genetics* O Antigens / metabolism Reverse Transcriptase Polymerase Chain Reaction Sequence Analysis, DNA Temperature Transcription, Genetic |
| IF | 3.006 |
| 引用数 | 19 |
| WOS 分野 | MICROBIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 一般微生物 | JCM 3980 |