論文 - 詳細
| RRC ID | 15671 |
|---|---|
| 著者 | Liu D, Cai J, Yao T, Wang Y, Kong Q, Feng Y. |
| タイトル | [Isolation and identification of a thermophilic bacterium from hot springs in Changbai Mountains]. |
| ジャーナル | Wei Sheng Wu Xue Bao |
| Abstract |
OBJECTIVE:We studied physiological and biochemical properties of a thermophilic bacterium isolated from hot springs in Changbai Mountain. METHODS:The strain CBS-5 (=JCM 15484) from hot springs in Changbai Mountains was isolated by plating and screening on Olive-rich medium. It was characterized by the physiological and biochemical analysis, 16S rDNA sequencing and determination of (G+C)mol% contents. RESULTS:The cells were Gram-positive, non-motile rods, spore-forming and generally occurred singly or in pairs. The growth temperature ranges from 48 degrees C to 70 degrees C, optimum at 65 degrees C; growth pH ranges from 6.5 to 8.5, optimum at 7.7. The strain used glucose, sucrose, maltose, lactose and rhanmose as carbon and energy sources. The G+C content of DNA was 41.9 mol%. The major cellular fatty acids were iso-C15:0, C16:0 and iso-C17:0, respectively representing about 24.20%, 20.45% and 17.42% of total fatty acids. The 16S rDNA sequence analysis showed that the strain belonged to the Anoxybacillus genus, for the sequence similarity was 95.1%-98.5% to other known species of Anoxybacillus. CONCLUSION:Strain CBS-5 was a member of Anoxybacillus. |
| 巻・号 | 48(10) |
| ページ | 1285-9 |
| 公開日 | 2008-10-1 |
| PMID | 19160805 |
| MeSH | Bacillaceae / classification Bacillaceae / genetics Bacillaceae / isolation & purification* China DNA, Bacterial / genetics DNA, Ribosomal / genetics Hot Springs / microbiology* Hot Temperature Molecular Sequence Data Phylogeny RNA, Ribosomal, 16S / genetics |
| リソース情報 | |
| 一般微生物 | JCM 15484 |