| RRC ID |
15698
|
| Author |
Satoh R, Tanaka A, Kita A, Morita T, Matsumura Y, Umeda N, Takada M, Hayashi S, Tani T, Shinmyozu K, Sugiura R.
|
| Title |
Role of the RNA-binding protein Nrd1 in stress granule formation and its implication in the stress response in fission yeast.
|
| Journal |
PLoS One
|
| Abstract |
We have previously identified the RNA recognition motif (RRM)-type RNA-binding protein Nrd1 as an important regulator of the posttranscriptional expression of myosin in fission yeast. Pmk1 MAPK-dependent phosphorylation negatively regulates the RNA-binding activity of Nrd1. Here, we report the role of Nrd1 in stress-induced RNA granules. Nrd1 can localize to poly(A)-binding protein (Pabp)-positive RNA granules in response to various stress stimuli, including heat shock, arsenite treatment, and oxidative stress. Interestingly, compared with the unphosphorylatable Nrd1, Nrd1(DD) (phosphorylation-mimic version of Nrd1) translocates more quickly from the cytoplasm to the stress granules in response to various stimuli; this suggests that the phosphorylation of Nrd1 by MAPK enhances its localization to stress-induced cytoplasmic granules. Nrd1 binds to Cpc2 (fission yeast RACK) in a phosphorylation-dependent manner and deletion of Cpc2 affects the formation of Nrd1-positive granules upon arsenite treatment. Moreover, the depletion of Nrd1 leads to a delay in Pabp-positive RNA granule formation, and overexpression of Nrd1 results in an increased size and number of Pabp-positive granules. Interestingly, Nrd1 deletion induced resistance to sustained stresses and enhanced sensitivity to transient stresses. In conclusion, our results indicate that Nrd1 plays a role in stress-induced granule formation, which affects stress resistance in fission yeast.
|
| Volume |
7(1)
|
| Pages |
e29683
|
| Published |
2012-1-1
|
| DOI |
10.1371/journal.pone.0029683
|
| PII |
PONE-D-11-16151
|
| PMID |
22276125
|
| PMC |
PMC3261880
|
| MeSH |
Arsenites / pharmacology
Cadmium Chloride / pharmacology
Hydrogen Peroxide / pharmacology
Potassium Chloride / pharmacology
Protein Binding / drug effects
RNA, Fungal / metabolism
Receptors for Activated C Kinase
Receptors, Cell Surface / metabolism
Ribonucleoproteins / metabolism*
Schizosaccharomyces / drug effects
Schizosaccharomyces / metabolism*
Schizosaccharomyces pombe Proteins / metabolism*
Sodium Compounds / pharmacology
Temperature
|
| IF |
2.74
|
| Times Cited |
13
|
|
WOS Category
|
CELL BIOLOGY
|
| Resource |
| Yeast |
HM123 |
