論文 - 詳細
| RRC ID | 15698 |
|---|---|
| 著者 | Satoh R, Tanaka A, Kita A, Morita T, Matsumura Y, Umeda N, Takada M, Hayashi S, Tani T, Shinmyozu K, Sugiura R. |
| タイトル | Role of the RNA-binding protein Nrd1 in stress granule formation and its implication in the stress response in fission yeast. |
| ジャーナル | PLoS One |
| Abstract |
We have previously identified the RNA recognition motif (RRM)-type RNA-binding protein Nrd1 as an important regulator of the posttranscriptional expression of myosin in fission yeast. Pmk1 MAPK-dependent phosphorylation negatively regulates the RNA-binding activity of Nrd1. Here, we report the role of Nrd1 in stress-induced RNA granules. Nrd1 can localize to poly(A)-binding protein (Pabp)-positive RNA granules in response to various stress stimuli, including heat shock, arsenite treatment, and oxidative stress. Interestingly, compared with the unphosphorylatable Nrd1, Nrd1(DD) (phosphorylation-mimic version of Nrd1) translocates more quickly from the cytoplasm to the stress granules in response to various stimuli; this suggests that the phosphorylation of Nrd1 by MAPK enhances its localization to stress-induced cytoplasmic granules. Nrd1 binds to Cpc2 (fission yeast RACK) in a phosphorylation-dependent manner and deletion of Cpc2 affects the formation of Nrd1-positive granules upon arsenite treatment. Moreover, the depletion of Nrd1 leads to a delay in Pabp-positive RNA granule formation, and overexpression of Nrd1 results in an increased size and number of Pabp-positive granules. Interestingly, Nrd1 deletion induced resistance to sustained stresses and enhanced sensitivity to transient stresses. In conclusion, our results indicate that Nrd1 plays a role in stress-induced granule formation, which affects stress resistance in fission yeast. |
| 巻・号 | 7(1) |
| ページ | e29683 |
| 公開日 | 2012-1-1 |
| DOI | 10.1371/journal.pone.0029683 |
| PII | PONE-D-11-16151 |
| PMID | 22276125 |
| PMC | PMC3261880 |
| MeSH | Arsenites / pharmacology Cadmium Chloride / pharmacology Hydrogen Peroxide / pharmacology Potassium Chloride / pharmacology Protein Binding / drug effects RNA, Fungal / metabolism Receptors for Activated C Kinase Receptors, Cell Surface / metabolism Ribonucleoproteins / metabolism* Schizosaccharomyces / drug effects Schizosaccharomyces / metabolism* Schizosaccharomyces pombe Proteins / metabolism* Sodium Compounds / pharmacology Temperature |
| IF | 2.74 |
| 引用数 | 13 |
| WOS 分野 | CELL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
| リソース情報 | |
| 酵母 | HM123 |