論文 - 詳細
| RRC ID | 15709 |
|---|---|
| 著者 | Santos F, Teusink B, Molenaar D, van Heck M, Wels M, Sieuwerts S, de Vos WM, Hugenholtz J. |
| タイトル | Effect of amino acid availability on vitamin B12 production in Lactobacillus reuteri. |
| ジャーナル | Appl Environ Microbiol |
| Abstract |
Recent functional genomics and genome-scale modeling approaches indicated that B(12) production in Lactobacillus reuteri could be improved by optimization of the medium. Here we show that a series of systematic single-amino-acid omissions could significantly modulate the production of B(12) from nearly undetectable levels (with omission of isoleucine) to levels 20-fold higher than the levels previously reported (with omission of cysteine). Using cDNA microarray experiments, we analyzed the transcriptional response of L. reuteri to medium lacking cysteine. The results supported the observed high level of B(12) production and provided new avenues for future improvement of production of vitamin B(12). |
| 巻・号 | 75(12) |
| ページ | 3930-6 |
| 公開日 | 2009-6-1 |
| DOI | 10.1128/AEM.02487-08 |
| PII | AEM.02487-08 |
| PMID | 19376900 |
| PMC | PMC2698342 |
| MeSH | Amino Acids / metabolism* Culture Media / chemistry Gene Expression Profiling Lactobacillus reuteri / metabolism* Oligonucleotide Array Sequence Analysis Vitamin B 12 / biosynthesis* |
| IF | 4.016 |
| 引用数 | 21 |
| WOS 分野 | BIOTECHNOLOGY & APPLIED MICROBIOLOGY MICROBIOLOGY |
| リソース情報 | |
| 一般微生物 | JCM 1112 |