RRC ID 15712
Author Kuang Y, Jia H, Miyanaga K, Tanji Y.
Title Effect of milk on antibacterial activity of tetracycline against Escherichia coli and Staphylococcus aureus isolated from bovine mastitis.
Journal Appl Microbiol Biotechnol
Abstract The susceptibility of mastitis-causing Escherichia coli and Staphylococcus aureus to two commonly used antibiotics, tetracycline and penicillin G, was tested in raw milk and in Muller-Hinton (MH) broth by introducing a pH indicator, bromocresol purple, which was shown to be a simple, sensitive, and rapid method. The minimum inhibitory concentration (MIC) of penicillin G in milk was the same as those in MH broth, whereas the MIC of tetracycline in milk was 4 to 32 times that in MH. An irreversible binding between tetracycline and large molecules of milk, which might be due to a hydrophobic interaction, was demonstrated by a dialysis test, suggesting the observed impairing effect was due to the action of milk on the tetracycline being tested. Further investigation revealed that much of the reduction of tetracycline's activity in milk was attributable to the milk protein casein, while other heat-sensitive components in milk also play some roles.
Volume 84(1)
Pages 135-42
Published 2009-8
DOI 10.1007/s00253-009-2008-6
PMID 19418049
MeSH Animals Anti-Bacterial Agents / antagonists & inhibitors* Anti-Bacterial Agents / pharmacology Cattle Escherichia coli / drug effects* Escherichia coli / isolation & purification Escherichia coli Infections / drug therapy Escherichia coli Infections / microbiology Escherichia coli Infections / veterinary* Female Mastitis, Bovine / drug therapy Mastitis, Bovine / microbiology* Milk / adverse effects* Milk / chemistry Protein Binding Staphylococcal Infections / drug therapy Staphylococcal Infections / microbiology Staphylococcal Infections / veterinary* Staphylococcus aureus / drug effects* Staphylococcus aureus / isolation & purification Tetracycline / antagonists & inhibitors* Tetracycline / pharmacology
IF 3.67
Times Cited 19
General Microbes JCM 2151