論文 - 詳細
| RRC ID | 15723 |
|---|---|
| 著者 | Saegusa S, Totsuka M, Kaminogawa S, Hosoi T. |
| タイトル | Saccharomyces cerevisiae and Candida albicans stimulate cytokine secretion from human neutrophil-like HL-60 cells differentiated with retinoic acid or dimethylsulfoxide. |
| ジャーナル | Biosci Biotechnol Biochem |
| Abstract |
We investigated whether non-pathogenic Saccharomyces cerevisiae and human commensal opportunistic pathogenic Candida albicans stimulate cytokine responses of human neutrophil-like HL-60 cells pre-treated with either 1 microM retinoic acid or 1.25% dimethyl sulfoxide (DMSO). Intact and heat-killed S. cerevisiae enhanced secretion of interleukin (IL)-1beta, IL-6, IL-8, IL-12, IL-18, MCP-1/CCL2 and TNF-alpha from retinoic acid-treated HL-60 cells, accompanied by alterations in mRNA expression of the cytokines. Heat-killed C. albicans promoted secretion of IL-6, IL-8, IL-12, MCP-1 and TNF-alpha, while intact C. albicans slightly enhanced secretion of IL-1beta, IL-8 and IL-18. In response to yeast stimuli, retinoic acid-treated HL-60 cells generally secreted cytokines more strongly than DMSO-treated HL-60 cells. Gene expression levels of Toll-like receptor (TLR)1, TLR2, TLR4, TLR6 and dectin-1 in HL-60 cells were additionally affected by retinoic acid or DMSO and by co-culturing with S. cerevisiae or C. albicans. Our results suggest that both intact and heat-killed S. cerevisiae and C. albicans induce cytokine responses of neutrophils in the intestine, and stimulate host immune function. |
| 巻・号 | 73(12) |
| ページ | 2600-8 |
| 公開日 | 2009-12-1 |
| DOI | 10.1271/bbb.90410 |
| PII | JST.JSTAGE/bbb/90410 |
| PMID | 19966493 |
| MeSH | Candida albicans / immunology* Candida albicans / metabolism Cell Differentiation / drug effects* Cytokines / genetics Cytokines / metabolism* Dimethyl Sulfoxide / pharmacology* Gene Expression Regulation / drug effects Gene Expression Regulation / immunology HL-60 Cells Hot Temperature Humans Lectins, C-Type Membrane Proteins / genetics Membrane Proteins / metabolism Nerve Tissue Proteins / genetics Nerve Tissue Proteins / metabolism Neutrophils / cytology Neutrophils / drug effects* Neutrophils / immunology Neutrophils / metabolism RNA, Messenger / genetics RNA, Messenger / metabolism Saccharomyces cerevisiae / immunology* Saccharomyces cerevisiae / metabolism Toll-Like Receptors / genetics Toll-Like Receptors / metabolism Tretinoin / pharmacology* |
| IF | 1.516 |
| 引用数 | 12 |
| WOS 分野 | FOOD SCIENCE & TECHNOLOGY CHEMISTRY, APPLIED BIOTECHNOLOGY & APPLIED MICROBIOLOGY BIOCHEMISTRY & MOLECULAR BIOLOGY |
| リソース情報 | |
| ヒト・動物細胞 | HL60(RCB0041) |
| 一般微生物 | JCM 1542 JCM 7255 |