Reference - Detail
RRC ID | 15726 |
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Author | Liu B, Wu L, Liu T, Hong Y, Shen Y, Ni J. |
Title | A MOFRL family glycerate kinase from the thermophilic crenarchaeon, Sulfolobus tokodaii, with unique enzymatic properties. |
Journal | Biotechnol Lett |
Abstract |
A glycerate kinase gene (ST2037) from the hyperthermophilic crenarchaeon Sulfolobus tokodaii was cloned and expressed in Escherichia coli. The purified homodimeric protein (45 kDa) specifically catalyzed the formation of 2-phosphoglycerate with D-glycerate as substrate. The thermostable enzyme displayed maximum activity (over 20 min) at 90 degrees C and pH 4.5. The maximal activity was in the presence of Co(2+). The MOFRL family glycerate kinase used AMP as phosphate donor with maximal activity towards GTP. These characteristics of the enzyme suggested its potential in the catalytic production of 2-phosphoglycerate. |
Volume | 31(12) |
Pages | 1937-41 |
Published | 2009-12-1 |
DOI | 10.1007/s10529-009-0089-z |
PMID | 19690808 |
MeSH | Adenosine Monophosphate / metabolism Cloning, Molecular Cobalt / pharmacology Coenzymes / pharmacology Dimerization Enzyme Stability Escherichia coli / genetics Gene Expression Glyceric Acids / metabolism Guanosine Triphosphate / metabolism Hydrogen-Ion Concentration Molecular Weight Phosphotransferases (Alcohol Group Acceptor) / chemistry Phosphotransferases (Alcohol Group Acceptor) / genetics* Phosphotransferases (Alcohol Group Acceptor) / isolation & purification Phosphotransferases (Alcohol Group Acceptor) / metabolism* Recombinant Proteins / chemistry Recombinant Proteins / genetics Recombinant Proteins / isolation & purification Recombinant Proteins / metabolism Sulfolobus / enzymology* Sulfolobus / genetics* Temperature |
IF | 1.977 |
Times Cited | 4 |
WOS Category | BIOTECHNOLOGY & APPLIED MICROBIOLOGY |
Resource | |
General Microbes | JCM 10545 |