論文 - 詳細
| RRC ID | 15727 |
|---|---|
| 著者 | Yokobori S, Itoh T, Yoshinari S, Nomura N, Sako Y, Yamagishi A, Oshima T, Kita K, Watanabe Y. |
| タイトル | Gain and loss of an intron in a protein-coding gene in Archaea: the case of an archaeal RNA pseudouridine synthase gene. |
| ジャーナル | BMC Evol Biol |
| Abstract |
BACKGROUND:We previously found the first examples of splicing of archaeal pre-mRNAs for homologs of the eukaryotic CBF5 protein (also known as dyskerin in humans) in Aeropyrum pernix, Sulfolobus solfataricus, S. tokodaii, and S. acidocaldarirus, and also showed that crenarchaeal species in orders Desulfurococcales and Sulfolobales, except for Hyperthermus butylicus, Pyrodictium occultum, Pyrolobus fumarii, and Ignicoccus islandicus, contain the (putative) cbf5 intron. However, the exact timing of the intron insertion was not determined and verification of the putative secondary loss of the intron in some lineages was not performed. RESULTS:In the present study, we determined approximately two-thirds of the entire coding region of crenarchaeal Cbf5 sequences from 43 species. A phylogenetic analysis of our data and information from the available genome sequences suggested that the (putative) cbf5 intron existed in the common ancestor of the orders Desulfurococcales and Sulfolobales and that probably at least two independent lineages in the order Desulfurococcales lost the (putative) intron. CONCLUSION:This finding is the first observation of a lineage-specific loss of a pre-mRNA intron in Archaea. As the insertion or deletion of introns in protein-coding genes in Archaea has not yet been seriously considered, our finding suggests the possible difficulty of accurately and completely predicting protein-coding genes in Archaea. |
| 巻・号 | 9 |
| ページ | 198 |
| 公開日 | 2009-8-11 |
| DOI | 10.1186/1471-2148-9-198 |
| PII | 1471-2148-9-198 |
| PMID | 19671140 |
| PMC | PMC2738675 |
| MeSH | Archaea / enzymology Archaea / genetics* Archaeal Proteins / genetics Base Sequence Evolution, Molecular Genes, Archaeal Intramolecular Transferases / genetics* Introns* Molecular Sequence Data Phylogeny RNA Precursors / genetics RNA, Archaeal / genetics* Sequence Alignment Sequence Analysis, RNA |
| IF | 3.058 |
| 引用数 | 15 |
| WOS 分野 | GENETICS & HEREDITY EVOLUTIONARY BIOLOGY |
| リソース情報 | |
| 一般微生物 | JCM 8857 JCM 8931 JCM 8954 JCM 8955 JCM 9021 JCM 9063 JCM 9065 JCM 9184 JCM 9185 JCM 9186 JCM 9187 JCM 9188 JCM 9189 JCM 9190 JCM 9191 JCM 9277 JCM 9278 JCM 9392 JCM 9393 JCM 9394 JCM 9403 JCM 9404 JCM 9630 JCM 9820 JCM 10088 JCM 10135 JCM 10307 JCM 10309 JCM 10545 JCM 10595 JCM 10830 JCM11212 JCM 11219 JCM 11320 JCM 11321 JCM 11322 JCM 11548 JCM 11597 JCM 11604 JCM 12091 JCM 13409 JCM 13516 |