RRC ID 15775
著者 Tanioka Y, Miyamoto E, Yabuta Y, Ohnishi K, Fujita T, Yamaji R, Misono H, Shigeoka S, Nakano Y, Inui H, Watanabe F.
タイトル Methyladeninylcobamide functions as the cofactor of methionine synthase in a Cyanobacterium, Spirulina platensis NIES-39.
ジャーナル FEBS Lett
Abstract To clarify the physiological function of pseudovitamin B(12) (or adeninylcobamide; AdeCba) in Spirulina platensis NIES-39, cobalamin-dependent methionine synthase (MS) was characterized. We cloned the full-length Spirulina MS. The clone contained an open reading frame encoding a protein of 1183 amino acids with a molecular mass of 132 kDa. Deduced amino acid sequences of the Spirulina MS contained critical residues identical to cobalamin-, zinc-, S-adenosylmethionine-, and homocysteine-binding motifs. The recombinant Spirulina enzyme showed higher affinity for methyladeninylcobamide than methylcobalamin as a cofactor. These results indicate that Spirulina cells can utilize AdeCba synthesized as the cofactor for MS.
巻・号 584(14)
ページ 3223-6
公開日 2010-7-16
DOI 10.1016/j.febslet.2010.06.013
PII S0014-5793(10)00503-X
PMID 20558164
MeSH 5-Methyltetrahydrofolate-Homocysteine S-Methyltransferase / chemistry 5-Methyltetrahydrofolate-Homocysteine S-Methyltransferase / genetics 5-Methyltetrahydrofolate-Homocysteine S-Methyltransferase / metabolism* Amino Acid Sequence Bacteria / genetics Bacteria / metabolism Cyanobacteria / genetics Cyanobacteria / metabolism* Inorganic Chemicals Molecular Sequence Data Organic Chemicals Protein Structure, Tertiary / genetics S-Adenosylmethionine / genetics S-Adenosylmethionine / metabolism Vitamin B 12 / analogs & derivatives Vitamin B 12 / genetics Vitamin B 12 / metabolism
IF 3.057
引用数 20
WOS 分野 BIOPHYSICS BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY
リソース情報
一般微生物 JCM 6427
藻類 NIES-39