論文 - 詳細
RRC ID | 15775 |
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著者 | Tanioka Y, Miyamoto E, Yabuta Y, Ohnishi K, Fujita T, Yamaji R, Misono H, Shigeoka S, Nakano Y, Inui H, Watanabe F. |
タイトル | Methyladeninylcobamide functions as the cofactor of methionine synthase in a Cyanobacterium, Spirulina platensis NIES-39. |
ジャーナル | FEBS Lett |
Abstract |
To clarify the physiological function of pseudovitamin B(12) (or adeninylcobamide; AdeCba) in Spirulina platensis NIES-39, cobalamin-dependent methionine synthase (MS) was characterized. We cloned the full-length Spirulina MS. The clone contained an open reading frame encoding a protein of 1183 amino acids with a molecular mass of 132 kDa. Deduced amino acid sequences of the Spirulina MS contained critical residues identical to cobalamin-, zinc-, S-adenosylmethionine-, and homocysteine-binding motifs. The recombinant Spirulina enzyme showed higher affinity for methyladeninylcobamide than methylcobalamin as a cofactor. These results indicate that Spirulina cells can utilize AdeCba synthesized as the cofactor for MS. |
巻・号 | 584(14) |
ページ | 3223-6 |
公開日 | 2010-7-16 |
DOI | 10.1016/j.febslet.2010.06.013 |
PII | S0014-5793(10)00503-X |
PMID | 20558164 |
MeSH | 5-Methyltetrahydrofolate-Homocysteine S-Methyltransferase / chemistry 5-Methyltetrahydrofolate-Homocysteine S-Methyltransferase / genetics 5-Methyltetrahydrofolate-Homocysteine S-Methyltransferase / metabolism* Amino Acid Sequence Bacteria / genetics Bacteria / metabolism Cyanobacteria / genetics Cyanobacteria / metabolism* Inorganic Chemicals Molecular Sequence Data Organic Chemicals Protein Structure, Tertiary / genetics S-Adenosylmethionine / genetics S-Adenosylmethionine / metabolism Vitamin B 12 / analogs & derivatives Vitamin B 12 / genetics Vitamin B 12 / metabolism |
IF | 3.057 |
引用数 | 20 |
WOS 分野 | BIOPHYSICS BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY |
リソース情報 | |
一般微生物 | JCM 6427 |
藻類 | NIES-39 |