| RRC ID |
15808
|
| 著者 |
Everest GJ, Cook AE, Kirby BM, Meyers PR.
|
| タイトル |
Evaluation of the use of recN sequence analysis in the phylogeny of the genus Amycolatopsis.
|
| ジャーナル |
Antonie Van Leeuwenhoek
|
| Abstract |
Partial recN gene sequences (>1 kb) were obtained from 35 type strains of the genus Amycolatopsis. Phylogenetic trees were constructed to determine the effectiveness of using this gene to predict taxonomic relationships within the genus. The use of recN sequence analysis as an alternative to DNA-DNA hybridization (DDH) for distinguishing closely related species was also assessed. The recN based phylogeny mostly confirmed the conventional 16S rRNA and gyrB gene-based phylogenies and thus provides further support for these phylogenetic groupings. As is the case for the gyrB gene, pairwise recN sequence similarities cannot be used to predict the DNA relatedness between type strains but the recN genetic distance can be used as a means to assess quickly whether an isolate is likely to represent a new species in the genus Amycolatopsis. A recN genetic distance of >0.04 between two Amycolatopsis strains is proposed to provide a good indication that they belong to different species (and that polyphasic taxonomic characterization of the unknown strain is worth undertaking).
|
| 巻・号 |
100(4)
|
| ページ |
483-96
|
| 公開日 |
2011-11-1
|
| DOI |
10.1007/s10482-011-9604-z
|
| PMID |
21671192
|
| MeSH |
Actinomycetales / classification*
Actinomycetales / enzymology
Actinomycetales / genetics
Actinomycetales / isolation & purification*
Bacterial Proteins / genetics*
Bacterial Typing Techniques / methods*
DNA Restriction Enzymes / genetics*
Molecular Sequence Data
Phylogeny*
|
| IF |
2.674
|
| 引用数 |
10
|
|
WOS 分野
|
MICROBIOLOGY
|
| リソース情報 |
| 一般微生物 |
JCM 21831
JCM 21832 |
