論文 - 詳細
| RRC ID | 15813 |
|---|---|
| 著者 | Masuda Y, Ono H, Kitagawa H, Ito H, Mu F, Sawa N, Zendo T, Sonomoto K. |
| タイトル | Identification and characterization of leucocyclicin Q, a novel cyclic bacteriocin produced by Leuconostoc mesenteroides TK41401. |
| ジャーナル | Appl Environ Microbiol |
| Abstract |
The culture supernatant of Leuconostoc mesenteroides TK41401, isolated from Japanese pickles, possessed antimicrobial activity against broad range of a bacterial genera and particularly strong activity against Bacillus coagulans, the major contaminant of pickles. An antimicrobial peptide was purified in three chromatographic steps, and its molecular mass was determined to be 6,115.59 Da by electrospray ionization time-of-flight mass spectrometry (ESI-TOF MS). The primary structure of this peptide was determined by amino acid and DNA sequencing, and these analyses revealed that it was translated as a 63-residue precursor. This precursor showed high similarity to the precursor of lactocyclicin Q, a cyclic bacteriocin produced by Lactococcus sp. strain QU 12. The molecular weight calculated after cyclization, which was presumed to involve the same process as in lactocyclicin Q (between L3 and W63), agreed with that estimated by ESI-TOF MS. This peptide was proved to be a novel cyclic bacteriocin, and it was termed leucocyclicin Q. The antimicrobial spectrum of this bacteriocin clearly differed from that of lactocyclicin Q, even though their primary structures were quite similar. This is the first report of a cyclic bacteriocin produced by a strain of the genus Leuconostoc. |
| 巻・号 | 77(22) |
| ページ | 8164-70 |
| 公開日 | 2011-11-1 |
| DOI | 10.1128/AEM.06348-11 |
| PII | AEM.06348-11 |
| PMID | 21948835 |
| PMC | PMC3209004 |
| MeSH | Amino Acid Sequence Anti-Bacterial Agents / chemistry Anti-Bacterial Agents / isolation & purification Anti-Bacterial Agents / metabolism* Bacillus / drug effects Bacteriocins / chemistry Bacteriocins / genetics* Bacteriocins / isolation & purification Bacteriocins / metabolism* Base Sequence Chromatography, Liquid Culture Media / chemistry DNA, Bacterial / chemistry DNA, Bacterial / genetics Food Microbiology Leuconostoc / genetics* Leuconostoc / isolation & purification Leuconostoc / metabolism* Molecular Sequence Data Molecular Weight Peptides / chemistry Peptides / genetics Peptides / isolation & purification Peptides / metabolism Sequence Analysis, DNA Spectrometry, Mass, Electrospray Ionization |
| IF | 4.016 |
| 引用数 | 47 |
| WOS 分野 | BIOTECHNOLOGY & APPLIED MICROBIOLOGY MICROBIOLOGY |
| リソース情報 | |
| 一般微生物 | JCM 1157 JCM 1465 JCM 2152 JCM 2257 JCM 5705 JCM 5707 JCM 5802 JCM 5803 JCM 5804 JCM 5885 JCM 5887 JCM 6124 JCM 7638 JCM 9890 JCM 12495 |