| RRC ID |
15827
|
| Author |
Sawai H, Na K, Sasaki N, Mimitsuka T, Minegishi S, Henmi M, Yamada K, Shimizu S, Yonehara T.
|
| Title |
Membrane-integrated fermentation system for improving the optical purity of D-lactic acid produced during continuous fermentation.
|
| Journal |
Biosci Biotechnol Biochem
|
| Abstract |
This report describes the production of highly optically pure D-lactic acid by the continuous fermentation of Sporolactobacillus laevolacticus and S. inulinus, using a membrane-integrated fermentation (MFR) system. The optical purity of D-lactic acid produced by the continuous fermentation system was greater than that produced by batch fermentation; the maximum value for the optical purity of D-lactic acid reached 99.8% enantiomeric excess by continuous fermentation when S. leavolacticus was used. The volumetric productivity of the optically pure D-lactic acid was about 12 g/L/h, this being approximately 11-fold higher than that obtained by batch fermentation. An enzymatic analysis indicated that both S. laevolacticus and S. inulinus could convert L-lactic acid to D-lactic acid by isomerization after the late-log phase. These results provide evidence for an effective bio-process to produce D-lactic acid of greater optical purity than has conventionally been achieved to date.
|
| Volume |
75(12)
|
| Pages |
2326-32
|
| Published |
2011-1-1
|
| DOI |
10.1271/bbb.110486
|
| PII |
JST.JSTAGE/bbb/110486
|
| PMID |
22146719
|
| MeSH |
Bacillales / cytology*
Bacillales / enzymology
Bacillales / metabolism*
Bioreactors / microbiology*
Cell Membrane / metabolism*
Fermentation*
L-Lactate Dehydrogenase / metabolism
Lactic Acid / biosynthesis*
Lactic Acid / chemistry
Lactobacillus / metabolism
Optical Phenomena*
Stereoisomerism
|
| IF |
1.516
|
| Times Cited |
11
|
|
WOS Category
|
FOOD SCIENCE & TECHNOLOGY
BIOTECHNOLOGY & APPLIED MICROBIOLOGY
CHEMISTRY, APPLIED
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| Resource |
| General Microbes |
JCM 1012
JCM 1164
JCM 2513
JCM 2515
JCM 6014
JCM 6124 |
