論文 - 詳細
| RRC ID | 15892 |
|---|---|
| 著者 | Shimane Y, Hatada Y, Minegishi H, Echigo A, Nagaoka S, Miyazaki M, Ohta Y, Maruyama T, Usami R, Grant WD, Horikoshi K. |
| タイトル | Salarchaeum japonicum gen. nov., sp. nov., an aerobic, extremely halophilic member of the Archaea isolated from commercial salt. |
| ジャーナル | Int J Syst Evol Microbiol |
| Abstract |
Strain YSM-79(T) was isolated from commercial salt made from seawater in Yonaguni island, Okinawa, Japan. The strain is an aerobic, Gram-negative, chemo-organotrophic and extremely halophilic archaeon. Cells are short rods that lyse in distilled water. Growth occurs at 1.5-5.3 M NaCl (optimum 2.5-3.0 M), pH 5.0-8.8 (optimum pH 5.2-6.3) and 20-55 °C (optimum 40 °C). Mg²⁺ is required for growth, with maximum growth at 200-300 mM Mg²⁺. Polar lipid analysis revealed the presence of phosphatidylglycerol, phosphatidylglycerophosphate methyl ester, sulfated diglycosyl diether-1 and five unidentified glycolipids. The G+C content of the DNA was 64 mol%. On the basis of 16S rRNA gene sequence analysis, strain YSM-79(T) was determined to be a member of the family Halobacteriaceae, with the closest related genus being Halobacterium (94 % sequence identity). In addition, the rpoB' gene sequence of strain YSM-79(T) had <88 % sequence similarity to those of other members of the family Halobacteriaceae. The results of phenotypic, chemotaxonomic and phylogenetic analysis suggested that strain YSM-79(T) should be placed in a new genus, Salarchaeum gen. nov., as a representative of Salarchaeum japonicum sp. nov. The type strain is YSM-79(T) ( = JCM 16327(T) = CECT 7563(T)). |
| 巻・号 | 61(Pt 9) |
| ページ | 2266-2270 |
| 公開日 | 2011-9-1 |
| DOI | 10.1099/ijs.0.025064-0 |
| PMID | 20952548 |
| MeSH | Aerobiosis Base Composition Cluster Analysis DNA, Archaeal / chemistry DNA, Archaeal / genetics DNA, Ribosomal / chemistry DNA, Ribosomal / genetics DNA-Directed RNA Polymerases / genetics Food Microbiology* Genes, rRNA Halobacteriaceae / classification* Halobacteriaceae / genetics Halobacteriaceae / isolation & purification* Halobacteriaceae / physiology Hydrogen-Ion Concentration Japan Magnesium / metabolism Molecular Sequence Data Phospholipids / analysis Phylogeny RNA, Archaeal / genetics RNA, Ribosomal, 16S / genetics Salts* Sequence Analysis, DNA Sequence Homology, Nucleic Acid Sodium Chloride / metabolism Temperature |
| IF | 2.415 |
| 引用数 | 20 |
| WOS 分野 | MICROBIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Wikipedia |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 一般微生物 | JCM 16327 |