| RRC ID |
15892
|
| Author |
Shimane Y, Hatada Y, Minegishi H, Echigo A, Nagaoka S, Miyazaki M, Ohta Y, Maruyama T, Usami R, Grant WD, Horikoshi K.
|
| Title |
Salarchaeum japonicum gen. nov., sp. nov., an aerobic, extremely halophilic member of the Archaea isolated from commercial salt.
|
| Journal |
Int J Syst Evol Microbiol
|
| Abstract |
Strain YSM-79(T) was isolated from commercial salt made from seawater in Yonaguni island, Okinawa, Japan. The strain is an aerobic, Gram-negative, chemo-organotrophic and extremely halophilic archaeon. Cells are short rods that lyse in distilled water. Growth occurs at 1.5-5.3 M NaCl (optimum 2.5-3.0 M), pH 5.0-8.8 (optimum pH 5.2-6.3) and 20-55 °C (optimum 40 °C). Mg²⁺ is required for growth, with maximum growth at 200-300 mM Mg²⁺. Polar lipid analysis revealed the presence of phosphatidylglycerol, phosphatidylglycerophosphate methyl ester, sulfated diglycosyl diether-1 and five unidentified glycolipids. The G+C content of the DNA was 64 mol%. On the basis of 16S rRNA gene sequence analysis, strain YSM-79(T) was determined to be a member of the family Halobacteriaceae, with the closest related genus being Halobacterium (94 % sequence identity). In addition, the rpoB' gene sequence of strain YSM-79(T) had <88 % sequence similarity to those of other members of the family Halobacteriaceae. The results of phenotypic, chemotaxonomic and phylogenetic analysis suggested that strain YSM-79(T) should be placed in a new genus, Salarchaeum gen. nov., as a representative of Salarchaeum japonicum sp. nov. The type strain is YSM-79(T) ( = JCM 16327(T) = CECT 7563(T)).
|
| Volume |
61(Pt 9)
|
| Pages |
2266-2270
|
| Published |
2011-9-1
|
| DOI |
10.1099/ijs.0.025064-0
|
| PMID |
20952548
|
| MeSH |
Aerobiosis
Base Composition
Cluster Analysis
DNA, Archaeal / chemistry
DNA, Archaeal / genetics
DNA, Ribosomal / chemistry
DNA, Ribosomal / genetics
DNA-Directed RNA Polymerases / genetics
Food Microbiology*
Genes, rRNA
Halobacteriaceae / classification*
Halobacteriaceae / genetics
Halobacteriaceae / isolation & purification*
Halobacteriaceae / physiology
Hydrogen-Ion Concentration
Japan
Magnesium / metabolism
Molecular Sequence Data
Phospholipids / analysis
Phylogeny
RNA, Archaeal / genetics
RNA, Ribosomal, 16S / genetics
Salts*
Sequence Analysis, DNA
Sequence Homology, Nucleic Acid
Sodium Chloride / metabolism
Temperature
|
| IF |
2.415
|
| Times Cited |
20
|
|
WOS Category
|
MICROBIOLOGY
|
| Resource |
| General Microbes |
JCM 16327 |
