RRC ID 15892
著者 Shimane Y, Hatada Y, Minegishi H, Echigo A, Nagaoka S, Miyazaki M, Ohta Y, Maruyama T, Usami R, Grant WD, Horikoshi K.
タイトル Salarchaeum japonicum gen. nov., sp. nov., an aerobic, extremely halophilic member of the Archaea isolated from commercial salt.
ジャーナル Int J Syst Evol Microbiol
Abstract Strain YSM-79(T) was isolated from commercial salt made from seawater in Yonaguni island, Okinawa, Japan. The strain is an aerobic, Gram-negative, chemo-organotrophic and extremely halophilic archaeon. Cells are short rods that lyse in distilled water. Growth occurs at 1.5-5.3 M NaCl (optimum 2.5-3.0 M), pH 5.0-8.8 (optimum pH 5.2-6.3) and 20-55 °C (optimum 40 °C). Mg²⁺ is required for growth, with maximum growth at 200-300 mM Mg²⁺. Polar lipid analysis revealed the presence of phosphatidylglycerol, phosphatidylglycerophosphate methyl ester, sulfated diglycosyl diether-1 and five unidentified glycolipids. The G+C content of the DNA was 64 mol%. On the basis of 16S rRNA gene sequence analysis, strain YSM-79(T) was determined to be a member of the family Halobacteriaceae, with the closest related genus being Halobacterium (94 % sequence identity). In addition, the rpoB' gene sequence of strain YSM-79(T) had <88 % sequence similarity to those of other members of the family Halobacteriaceae. The results of phenotypic, chemotaxonomic and phylogenetic analysis suggested that strain YSM-79(T) should be placed in a new genus, Salarchaeum gen. nov., as a representative of Salarchaeum japonicum sp. nov. The type strain is YSM-79(T) ( = JCM 16327(T)  = CECT 7563(T)).
巻・号 61(Pt 9)
ページ 2266-2270
公開日 2011-9-1
DOI 10.1099/ijs.0.025064-0
PMID 20952548
MeSH Aerobiosis Base Composition Cluster Analysis DNA, Archaeal / chemistry DNA, Archaeal / genetics DNA, Ribosomal / chemistry DNA, Ribosomal / genetics DNA-Directed RNA Polymerases / genetics Food Microbiology* Genes, rRNA Halobacteriaceae / classification* Halobacteriaceae / genetics Halobacteriaceae / isolation & purification* Halobacteriaceae / physiology Hydrogen-Ion Concentration Japan Magnesium / metabolism Molecular Sequence Data Phospholipids / analysis Phylogeny RNA, Archaeal / genetics RNA, Ribosomal, 16S / genetics Salts* Sequence Analysis, DNA Sequence Homology, Nucleic Acid Sodium Chloride / metabolism Temperature
IF 2.415
引用数 20
WOS 分野 MICROBIOLOGY
リソース情報
一般微生物 JCM 16327