RRC ID |
1594
|
著者 |
Zhang TY, Kang L, Zhang ZF, Xu WH.
|
タイトル |
Identification of a POU factor involved in regulating the neuron-specific expression of the gene encoding diapause hormone and pheromone biosynthesis-activating neuropeptide in Bombyx mori.
|
ジャーナル |
Biochem J
|
Abstract |
Diapause hormone (DH) and PBAN (pheromone biosynthesis-activating neuropeptide) are two important insect neuropeptides regulating development and reproduction respectively. In the present study, we report two Bombyx mori transcription factors interacting specifically with the promoter of Bom-DH-PBAN (where Bom-DH stands for B. mori DH); we named them DHMBP-1 and -2 (DH-modulator-binding proteins 1 and 2). The developmental changes of DHMBP-1/-2 are closely correlated with that of Bom-DH-PBAN mRNA throughout the pupal stage. Competition assays indicate that DHMBP-1 from Chinese B. mori possesses binding characteristics similar to those of the POU-M1 protein from Japanese B. mori. POU-M1 cDNAs were cloned from various tissues of Chinese B. mori and were found to be distinct from the previously published POU-M1 in amino acid residues 108-136 because of insertion mutations. Owing to this difference in amino acid residues, we named this cDNA POU-M2. Even though POU-M2 differs from POU-M1 at the N-terminal, the POU domain and the binding properties of both POU-M1 and -M2 are the same. Functional analysis showed that overexpression of POU-M2 in the Bombyx cell line BmN activated the promoter of Bom-DH-PBAN, but failed to activate a promoter in which the POU-binding element was mutated. The transcriptional activity of POU-M2 is probably regulated by other factors binding to the upstream of the promoter sequence. We show that the POU-M2-binding site was able to activate the transcription of a heterologous promoter of the gene encoding B. mori larval serum protein. POU-M1 was found to exhibit the same transcriptional activities as POU-M2. Taken together, these results demonstrate that POU-M2 plays an important role in the transcriptional regulation of the Bom-DH-PBAN gene.
|
巻・号 |
380(Pt 1)
|
ページ |
255-63
|
公開日 |
2004-5-15
|
DOI |
10.1042/BJ20031482
|
PII |
BJ20031482
|
PMID |
14766018
|
PMC |
PMC1224146
|
MeSH |
Amino Acid Sequence
Animals
Base Sequence
Bombyx / growth & development
Bombyx / metabolism*
DNA-Binding Proteins / chemistry
Gene Expression Regulation
Genes, Reporter
Insect Proteins / biosynthesis*
Insect Proteins / chemistry
Insect Proteins / genetics
Insect Proteins / isolation & purification*
Insect Proteins / physiology
Larva
Molecular Sequence Data
Nerve Tissue Proteins / biosynthesis*
Nerve Tissue Proteins / genetics
Neurons / metabolism*
Neuropeptides / biosynthesis*
Neuropeptides / genetics
POU Domain Factors
Promoter Regions, Genetic
Protein Precursors / biosynthesis*
Protein Precursors / genetics
Protein Structure, Tertiary
Pupa
Recombinant Fusion Proteins / physiology
Sequence Alignment
Sequence Homology
Species Specificity
Transcription Factors / chemistry
Transcription Factors / isolation & purification*
Transcription Factors / physiology
Transcription, Genetic
Transfection
|
IF |
4.097
|
引用数 |
36
|
WOS 分野
|
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
リソース情報 |
遺伝子材料 |
Bombyx POU-M1/SGF-3 (RDB01829) |