論文 - 詳細
| RRC ID | 1595 |
|---|---|
| 著者 | Arap MA, Lahdenranta J, Hajitou A, Marini FC 3rd, Wood CG, Wright KC, Fueyo J, Arap W, Pasqualini R. |
| タイトル | Model of unidirectional transluminal gene transfer. |
| ジャーナル | Mol Ther |
| Abstract |
Gene transfer assays in vitro are poor indicators of transduction efficacy observed in vivo. We designed and optimized an intermediate model for assessing and quantifying unidirectional transduction ex vivo. The model enables simultaneous transmucosal evaluation of up to 96 different variables under the same tissue conditions. We show that the model is versatile and suitable for use with different vectors (adenovirus and AAV), different reporter genes (beta-galactosidase and green fluorescent protein), and viscera with various tissue features such as peritoneum and urothelium. Ex vivo transduction assays may correlate better with in vivo gene transfer results. Because the experimental model described here can be performed in small samples, it may enable translational applications in tissues of human origin. |
| 巻・号 | 9(2) |
| ページ | 305-10 |
| 公開日 | 2004-2-1 |
| DOI | 10.1016/j.ymthe.2003.11.016 |
| PII | S1525-0016(03)00363-0 |
| PMID | 14759814 |
| MeSH | Animals Gene Expression Genes, Reporter / genetics Genetic Vectors / genetics Glycosaminoglycans / metabolism Humans In Vitro Techniques Peritoneum / metabolism Swine Transduction, Genetic / methods* Transduction, Genetic / standards Transgenes / genetics Ureter / metabolism |
| IF | 8.986 |
| 引用数 | 4 |
| WOS 分野 | MEDICINE, RESEARCH & EXPERIMENTAL BIOTECHNOLOGY & APPLIED MICROBIOLOGY GENETICS & HEREDITY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 遺伝子材料 | pSK II+[X-K]nbK20 (RDB01892) |