RRC ID 1595
Author Arap MA, Lahdenranta J, Hajitou A, Marini FC 3rd, Wood CG, Wright KC, Fueyo J, Arap W, Pasqualini R.
Title Model of unidirectional transluminal gene transfer.
Journal Mol. Ther.
Abstract Gene transfer assays in vitro are poor indicators of transduction efficacy observed in vivo. We designed and optimized an intermediate model for assessing and quantifying unidirectional transduction ex vivo. The model enables simultaneous transmucosal evaluation of up to 96 different variables under the same tissue conditions. We show that the model is versatile and suitable for use with different vectors (adenovirus and AAV), different reporter genes (beta-galactosidase and green fluorescent protein), and viscera with various tissue features such as peritoneum and urothelium. Ex vivo transduction assays may correlate better with in vivo gene transfer results. Because the experimental model described here can be performed in small samples, it may enable translational applications in tissues of human origin.
Volume 9(2)
Pages 305-10
Published 2004-2
DOI 10.1016/j.ymthe.2003.11.016
PII S1525-0016(03)00363-0
PMID 14759814
MeSH Animals Gene Expression Genes, Reporter / genetics Genetic Vectors / genetics Glycosaminoglycans / metabolism Humans In Vitro Techniques Peritoneum / metabolism Swine Transduction, Genetic / methods* Transduction, Genetic / standards Transgenes / genetics Ureter / metabolism
IF 7.008
Times Cited 4
WOS Category MEDICINE, RESEARCH & EXPERIMENTAL BIOTECHNOLOGY & APPLIED MICROBIOLOGY GENETICS & HEREDITY
Resource
DNA material pSK II+[X-K]nbK20 (RDB01892)