RRC ID 15964
Author Taganna JC, Quanico JP, Perono RM, Amor EC, Rivera WL.
Title Tannin-rich fraction from Terminalia catappa inhibits quorum sensing (QS) in Chromobacterium violaceum and the QS-controlled biofilm maturation and LasA staphylolytic activity in Pseudomonas aeruginosa.
Journal J Ethnopharmacol
Abstract AIM OF THE STUDY:The study aimed to test the activity of Terminalia catappa L. against bacterial quorum sensing (QS) in order to provide a potential scientific basis for the traditional use of leaf extracts of this plant as an antiseptic.
MATERIALS AND METHODS:The anti-QS activity of the methanolic leaf extract of Terminalia catappa was detected through the inhibition of the QS-controlled violacein pigment production in Chromobacterium violaceum. Fractions resulting from size-exclusion chromatography were assayed. The most active fraction was characterized through qualitative phytochemical detection methods. The effect of this fraction on known QS-controlled phenotypes in test strains was assessed.
RESULTS:The fraction with the highest activity (labeled as TCF12) was characterized to be tannin-rich. It specifically inhibited QS-controlled violacein production in Chromobacterium violaceum with 50% reduction achieved at 62.5 μg mL(-1) without significantly affecting growth up to about 962 μg mL(-1). The assessment of its effects on LasA activity of Pseudomonas aeruginosa ATCC 10145 found that the production of this virulence determinant is reduced in a concentration dependent manner with about 50% reduction at 62.5 μg mL(-1). Furthermore, it was found that TCF12 was able to inhibit the maturation of biofilms of Pseudomonas aeruginosa, a phenotype that has also been known to be QS-regulated.
CONCLUSION:Therefore, tannin-rich components of Terminalia catappa leaves are able to inhibit certain phenotypic expression of QS in the test strains used.
Volume 134(3)
Pages 865-71
Published 2011-4-12
DOI 10.1016/j.jep.2011.01.028
PII S0378-8741(11)00042-0
PMID 21291979
MeSH Biofilms* Chromatography, Gel Chromobacterium / drug effects* Chromobacterium / growth & development Chromobacterium / physiology Metalloproteases / physiology* Plant Extracts / pharmacology* Pseudomonas aeruginosa / physiology* Quorum Sensing / drug effects* Tannins / pharmacology* Terminalia / chemistry* Virulence Factors / physiology*
IF 3.69
Times Cited 62
General Microbes JCM 1249