| RRC ID |
16000
|
| 著者 |
Wirth JF, Snyder JC, Hochstein RA, Ortmann AC, Willits DA, Douglas T, Young MJ.
|
| タイトル |
Development of a genetic system for the archaeal virus Sulfolobus turreted icosahedral virus (STIV).
|
| ジャーナル |
Virology
|
| Abstract |
Our understanding of archaeal viruses has been limited by the lack of genetic systems for examining viral function. We describe the construction of an infectious clone for the archaeal virus Sulfolobus turreted icosahedral virus (STIV). STIV was isolated from a high temperature (82°C) acidic (pH 2.2) hot spring in Yellowstone National Park and replicates in the archaeal model organism Sulfolobus solfataricus (Rice et al., 2004). While STIV is one of most studied archaeal viruses, little is known about its replication cycle. The development of an STIV infectious clone allows for directed gene disruptions and detailed genetic analysis of the virus. The utility of the STIV infectious clone was demonstrated by gene disruption of STIV open reading frame (ORF) B116 which resulted in crippled virus replication, while disruption of ORFs A197, C381 and B345 was lethal for virus replication.
|
| 巻・号 |
415(1)
|
| ページ |
6-11
|
| 公開日 |
2011-6-20
|
| DOI |
10.1016/j.virol.2011.03.023
|
| PII |
S0042-6822(11)00148-6
|
| PMID |
21496857
|
| MeSH |
Archaeal Viruses / genetics*
Archaeal Viruses / isolation & purification
Archaeal Viruses / physiology
Archaeal Viruses / ultrastructure
Base Sequence
Blotting, Western
Cloning, Molecular
Escherichia coli / genetics
Escherichia coli / virology
Mutation
Open Reading Frames
Polymerase Chain Reaction
Sequence Analysis, DNA
Sulfolobus / genetics
Sulfolobus / isolation & purification
Sulfolobus / ultrastructure
Sulfolobus / virology*
United States
Viral Proteins / chemistry
Viral Proteins / genetics*
Virus Replication*
|
| IF |
2.819
|
| 引用数 |
22
|
|
WOS 分野
|
VIROLOGY
|
| リソース情報 |
| 一般微生物 |
JCM 10545 |
